Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 143 (1997), 3033-3044; DOI  10.1099/00221287-143-9-3033
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yaar, L.
Right arrow Articles by Koltint, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yaar, L.
Right arrow Articles by Koltint, Y.
Agricola
Right arrow Articles by Yaar, L.
Right arrow Articles by Koltint, Y.

A Candida albicans RAS-related gene (CaRSRl) is involved in budding, cell morphogenesis and hypha development

Liora Yaar, Moshe Mevarech and Yigal Koltint{dagger}

Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

Author for correspondence: Yigal Koltin. Tel: + 1 617 761 6804. Fax: + 1 617 679 7467. e-mail : koltin@mpi.com

ABSTRACT

Summary: Candida albicans, the most important human fungal pathogen, is a dimorphic fungus that can grow either as a yeast or as a hyphal form in response to medium conditions. A RAS-related C. albicans gene (CaRSRl) was isolated as a suppressor of a cdc24b bud-emergence mutation of the baker's yeast, Saccharomyces cerevisiae. The deduced protein encoded by CaRSRl is 248 amino acids long and 56% identical to that encoded by the 5. cerevisiae RSRl (BUDI) gene. Disruption of CaRSRl in C. albicans indicated that CaRSRl is involved in both yeast and hypha development. In the yeast phase, CaRSRl is required for normal (polar) bud site selection and is involved in cell morphogenesis; in the yeast-mycelial transition it is involved in germ tube emergence; and in the development of the hyphae it is involved in cell elongation. The disruption of CaRSRl leads to reduced virulence in both heterozygote and homozygote disruptants in a dose-dependent manner. The reduced virulence can be attributed to the reduced germination and shorter hyphae resulting from the disruption of CaRSRl.

Keywords: C. albicans morphogenesis, RSRl, BUD1, GTPase, yeast bud site selection

{dagger} Present address: Millennium Pharmaceuticals, Inc., 640 Memorial Drive, Cambridge, MA 02139, USA.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
A. Brand, A. Vacharaksa, C. Bendel, J. Norton, P. Haynes, M. Henry-Stanley, C. Wells, K. Ross, N. A. R. Gow, and C. A. Gale
An Internal Polarity Landmark Is Important for Externally Induced Hyphal Behaviors in Candida albicans
Eukaryot. Cell, April 1, 2008; 7(4): 712 - 720.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. R. Fortwendel, W. Zhao, R. Bhabhra, S. Park, D. S. Perlin, D. S. Askew, and J. C. Rhodes
A Fungus-Specific Ras Homolog Contributes to the Hyphal Growth and Virulence of Aspergillus fumigatus
Eukaryot. Cell, December 1, 2005; 4(12): 1982 - 1989.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
D. L. Hausauer, M. Gerami-Nejad, C. Kistler-Anderson, and C. A. Gale
Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p
Eukaryot. Cell, July 1, 2005; 4(7): 1273 - 1286.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Bauer, P. Knechtle, J. Wendland, H. Helfer, and P. Philippsen
A Ras-like GTPase Is Involved in Hyphal Growth Guidance in the Filamentous Fungus Ashbya gossypii
Mol. Biol. Cell, October 1, 2004; 15(10): 4622 - 4632.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Brand, D. M. MacCallum, A. J. P. Brown, N. A. R. Gow, and F. C. Odds
Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus
Eukaryot. Cell, August 1, 2004; 3(4): 900 - 909.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. L. vandenBerg, A. S. Ibrahim, J. E. Edwards Jr., K. A. Toenjes, and D. I. Johnson
Cdc42p GTPase Regulates the Budded-to-Hyphal-Form Transition and Expression of Hypha-Specific Transcripts in Candida albicans
Eukaryot. Cell, June 1, 2004; 3(3): 724 - 734.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Bassilana, J. Blyth, and R. A. Arkowitz
Cdc24, the GDP-GTP Exchange Factor for Cdc42, Is Required for Invasive Hyphal Growth of Candida albicans
Eukaryot. Cell, February 1, 2003; 2(1): 9 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. Santoni, R. Lucciarini, C. Amantini, J. Jacobelli, E. Spreghini, P. Ballarini, M. Piccoli, and A. Gismondi
Candida albicans Expresses a Focal Adhesion Kinase-Like Protein That Undergoes Increased Tyrosine Phosphorylation upon Yeast Cell Adhesion to Vitronectin and the EA.hy 926 Human Endothelial Cell Line
Infect. Immun., July 1, 2002; 70(7): 3804 - 3815.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Chandra, D. M. Kuhn, P. K. Mukherjee, L. L. Hoyer, T. McCormick, and M. A. Ghannoum
Biofilm Formation by the Fungal Pathogen Candida albicans: Development, Architecture, and Drug Resistance
J. Bacteriol., September 15, 2001; 183(18): 5385 - 5394.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Y. Jong, M. F. Stins, S.-H. Huang, S. H. M. Chen, and K. S. Kim
Traversal of Candida albicans across Human Blood-Brain Barrier In Vitro
Infect. Immun., July 1, 2001; 69(7): 4536 - 4544.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. S. Toledo, S. B. Levery, E. Suzuki, A. H. Straus, and H. K. Takahashi
Characterization of cerebrosides from the thermally dimorphic mycopathogen Histoplasma capsulatum: expression of 2-hydroxy fatty N-acyl (E)-{{Delta}}3-unsaturation correlates with the yeast-mycelium phase transition
Glycobiology, February 1, 2001; 11(2): 113 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. McLain, R. Ascanio, C. Baker, R. A. Strohaver, and J. W. Dolan
Undecylenic Acid Inhibits Morphogenesis of Candida albicans
Antimicrob. Agents Chemother., October 1, 2000; 44(10): 2873 - 2875.
[Abstract] [Full Text]

Home page
 MicrobiologyHome page
F. C. Odds, L. Van Nuffel, and N. A. R. Gow
Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host
Microbiology, August 1, 2000; 146(8): 1881 - 1889.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
M. S. Toledo, S. B. Levery, A. H. Straus, and H. K. Takahashi
Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii
J. Lipid Res., May 1, 2000; 41(5): 797 - 806.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
J. A. Calera, X.-J. Zhao, and R. Calderone
Defective Hyphal Development and Avirulence Caused by a Deletion of the SSK1 Response Regulator Gene in Candida albicans
Infect. Immun., February 1, 2000; 68(2): 518 - 525.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y Ayad-Durieux, P Knechtle, S Goff, F Dietrich, and P Philippsen
A PAK-like protein kinase is required for maturation of young hyphae and septation in the filamentous ascomycete Ashbya gossypii
J. Cell Sci., January 12, 2000; 113(24): 4563 - 4575.
[Abstract] [PDF]

Home page
 MicrobiologyHome page
A. B. Herrero, M. C. Lopez, L. Fernandez-Lago, and A. Dominguez
Candida albicans and Yarrowia lipolytica as alternative models for analysing budding patterns and germ tube formation in dimorphic fungi
Microbiology, October 1, 1999; 145(10): 2727 - 2737.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
D. I. Johnson
Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity
Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Lay, L. K. Henry, J. Clifford, Y. Koltin, C. E. Bulawa, and J. M. Becker
Altered Expression of Selectable Marker URA3 in Gene-Disrupted Candida albicans Strains Complicates Interpretation of Virulence Studies
Infect. Immun., November 1, 1998; 66(11): 5301 - 5306.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
F. V. Lunel, L. Licciardello, S. Stefani, H. A. Verbrugh, W. J. G. Melchers, J. F. G. M. Meis, S. Scherer, and A. van Belkum
Lack of Consistent Short Sequence Repeat Polymorphisms in Genetically Homologous Colonizing and Invasive Candida albicans Strains
J. Bacteriol., August 1, 1998; 180(15): 3771 - 3778.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1997 Society for General Microbiology.