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Microbiology 144 (1998), 167-175; DOI  10.1099/00221287-144-1-167
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Isoschizomers of the restriction endonuclease Taql (T/CGA) requiring different metal ion concentrations and having a range of thermal stabilities from Thermus species from different continents

Simon G. Welch1, Sameera Al-Awadhi1 and Ralph A. D. Williams1

Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary & Westfield College, University of London, Mile End Road, London E l 4NS, UK

ABSTRACT

Summary: One-hundred-and-fifty-two isolates of the genus Thermus, collected from hot springs on four continents, were screened for evidence of the presence of the thermophilic Type II restriction endonuclease Taql (T/CGA). The presence of isoschizomers of Taql in 27 of the isolates, originating from hot springs in New Zealand, Iceland, USA, Japan, mainland Portugal and the island of Sao Miguel in the Azores, is reported. Six of the Taql-containing isolates from diverse geographical locations, identified by means of DNA/DNA homology and 16S rRNA sequence alignment as belonging to the Thermus species T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus and T. brockianus, were selected for comparative studies. The Taql isoschizomers from each of the six isolates were partially purified. They differed in their magnesium ion requirements, isoelectric points, subunit molecular masses and thermal stability.

Author for correspondence: Simon G. Welch. Tel: + 44 171 982 6336. Fax: +44 181 983 0531. e-mail: sgwelch@qmw.ac.uk

Keywords: Taql, Thermus, thermostable, restriction endonuclease






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