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Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK
John Innes Centre, Norwich NR4 7UH, UK
Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany
FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany
ABSTRACT
Summary: Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single
35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.
Author for correspondence: Colin P. Smith. Tel: + 44 161 200 4183. Fax: +44 161 236 0409. e-mail: colin.smith@ umist.ac.uk
Present address: Public Health Research Institute, 455 First Avenue, NY 10016, USA.
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