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Microbiology 144 (1998), 193-199; DOI  10.1099/00221287-144-1-193
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Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Pei Pei Chong1, Sylvia M. Podmore1, Helen M. Kieser2, Matthias Redenbach3, Kürsad Turgay4,{dagger}, Mohamed Marahiel4, David A. Hopwood2 and Colin P. Smith1

Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK
John Innes Centre, Norwich NR4 7UH, UK
Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany
FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany

ABSTRACT

Summary: Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single ~ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

Author for correspondence: Colin P. Smith. Tel: + 44 161 200 4183. Fax: +44 161 236 0409. e-mail: colin.smith@ umist.ac.uk

Keywords: Streptomyces coelicolor, antibiotic biosynthesis, lipopeptide, gene disruption, peptide synthetase

{dagger} Present address: Public Health Research Institute, 455 First Avenue, NY 10016, USA.




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