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Department of Microbiology and Immunology, McGi I I University, 3775 University Street, Montreal, Quebec, Canada H3A 2B4
1 Author for correspondence: Michael S. DuBow. Tel: + 1 514 398 3926. Fax: + 1 514 398 7052.e-mail: msdubow@microirnm.mcgill.ca
ABSTRACT
SUMMARY: Operons encoding homologous arsenic-resistance determinants (ars) have been discovered in bacterial plasmids from Gram-positive and Gram-negative, organisms, as well as in the Escherichia coli chromosome. However, evidence for this arsenic-resistance determinant in the medically and environmentally important bacterial species Pseudomonas aeruginosa is conflicting. Here the identification of a P. aeruginosa chromosomal ars operon homologue via cloning and complementation of an E. coli ars mutant is reported. The P. aeruginosa chromosomal ars operon contains three potential ORFs encoding proteins with significant sequence similarity to those encoded by the arsR, arsB and arsC genes of the plasmid-based and E. coli chromosomal ars operons. The cloned P. aeruginosa chromosomal ars operon confers augmented resistance to arsenic and antimony oxyanions in an E. coli arsB mutant and in wild-type P. aeruginosa. Expression of the operon was induced by arsenite at the mRNA level. DNA sequences homologous with this operon were detected in some, but not all, species of the genus Pseudomonas, suggesting that its conservation follows their taxonomic-based evolution.
Present address: Biochemistry Department, McGill University, Drummond Street, Montreal, Quebec, Canada H3G 1Y6.
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