HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leret, V. Articles by Blanco, C. Search for Related Content PubMed PubMed Citation Articles by Leret, V. Articles by Blanco, C. Agricola Articles by Leret, V. Articles by Blanco, C.

pBLA8, from Brevibacterium linens, belongs to a Gram-positive subfamily of ColE2-related plasmids

Membranes et osmoregulationi 6026, Universite Rennes I, Avenue du General Leclerc, 35042 Rennes Cedex, France

¹ Membranes et osmoregulationi 6026, Universite Rennes I, Avenue du General Leclerc, 35042 Rennes Cedex, France

ABSTRACT

SUMMARY: A 3.1 kb DNA fragment from pBLA8, a Brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. Using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. This fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, RepA and RepB, both essential for replication. Based on structural similarities and a strong conservation of ori, RepA and RepB, pBLA8 was assigned to a new subfamily of the ColE2 plasmid family. This subfamily is distinguished by the requirement for two Rep proteins and the location of an ori site upstream of the mpAB operon. RepA is thought to encode primase activity, whereas RepB could be a DNA-binding protein. An Escherichia coli-B. linens shuttle vector, derived from pBLA8, was constructed. Its host spectrum was extended to Arthrobacter species.

This article has been cited by other articles:

				M. Yagura, S.-y. Nishio, H. Kurozumi, C.-f. Wang, and T. Itoh Anatomy of the Replication Origin of Plasmid ColE2-P9 J. Bacteriol., February 1, 2006; 188(3): 999 - 1010. [Abstract] [Full Text] [PDF]

				C. Sandu, C.-B. Chiribau, P. Sachelaru, and R. Brandsch Plasmids for Nicotine-Dependent and -Independent Gene Expression in Arthrobacter nicotinovorans and Other Arthrobacter Species Appl. Envir. Microbiol., December 1, 2005; 71(12): 8920 - 8924. [Abstract] [Full Text] [PDF]

				F. Amarita, M. Yvon, M. Nardi, E. Chambellon, J. Delettre, and P. Bonnarme Identification and Functional Analysis of the Gene Encoding Methionine-{gamma}-Lyase in Brevibacterium linens Appl. Envir. Microbiol., December 1, 2004; 70(12): 7348 - 7354. [Abstract] [Full Text] [PDF]