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Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut, Storrs, CT 06269-3089, USA
2Author for correspondence: S. J. Geary. Tel: + 1 860 486 0835. Fax: + 1 860 486 2794.e-mail: sgeary @canrl.cag.uconn.edu
ABSTRACT
SUMMARY: The identification of a gene (g8pA) from Mycoplasma gallisepticum with homology to the P I cytadherence gene of Mycoplasma pneumoniae is reported. The gapA gene is a 28 pORF encoding a protein with a molecular mass of 105 kDa. Nucleotide sequence analysis of the gapA gene revealed 45% homology t o the M. pneumoniae P I gene, 46% homology t o the Mycoplasma genitalium MgPa gene and 47% homology to the Mycoplasma pirum PI-likeprotein gene. It has a 64 mol% A+T content compared to 46,60 and 72 mol%respectively for the PI, MgPa and the Pl-like protein genes. As with the PI and MgPa genes, gapA is a central gene in a multi-gene operon, but unlike the P1 and MgPa genes, there is only a single copy of gapA in the genome. GapA is a trypsin-sensitive surface-exposed protein. Chicken tracheal-ring inhibition-of-attachment assays, using anti-GapA Fab fragments, resulted in 64% inhibition of attachment. These results indicated that GapA plays a rolein cytadherence of M. gallisepticum to host cells.
Present address: Department of Medicine, Division of Infectious Diseases, A-331 0 Medical Center North, Vanderbilt University, Nashville, TN 37232-2605, USA.
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