| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 OET, UK
Institute of Cell and Molecular Biology, The University of Edinburgh, Daniel Rutherford Building, King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK
Unilever Research Laboratory, Port Sunlight, Quarry Road East, Bebington, WirraI L63 3JW, UK
4Author for correspondence: Kevin A. Hughes. Tel: +44 1223 221400. Fax: +44 1223 362616.e-mail: k.hughes@bas.ac.uk
ABSTRACT
SUMMARY: Biof ilm bacteria Enterobecter agglomerans 53b and Serratia marcescens Serr were isolated from a food processing factory. A bacteriophage (SF153b), which could infect and lyse strain 53b, was isolated from sewage. This has been shown to possess a polysaccharide depolymerase enzyme specific for the exopolysaccharide (EPS) of strain 53b. Using batch culture and chemostat-linked Modified Robbins Device systems it was observed that SF1 53b could degrade the EPS of a mono-species biofilm (strain 53b) and infect the cells. The disruption of the biofilm by phage was a combination of EPS degradation by the depolymerase and infection and subsequent cell lysis by the phage. Strain Serr biofilms were not susceptible to the phage and the biofilm EPS was not degraded by the phage glycanase, with the result that the biofilm was unaffected by the addition of SF153b phage. Scanning electron microscopy confirmed that specific phage could extensively degrade susceptible biofilms and continue to infect biofilm bacteria whilst EPS degradation was occurring.
This article has been cited by other articles:
|
|
 |
|
  D. Schwarzer, K. Stummeyer, T. Haselhorst, F. Freiberger, B. Rode, M. Grove, T. Scheper, M. von Itzstein, M. Muhlenhoff, and R. Gerardy-Schahn Proteolytic Release of the Intramolecular Chaperone Domain Confers Processivity to Endosialidase F J. Biol. Chem., April 3, 2009; 284(14): 9465 - 9474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Briandet, P. Lacroix-Gueu, M. Renault, S. Lecart, T. Meylheuc, E. Bidnenko, K. Steenkeste, M.-N. Bellon-Fontaine, and M.-P. Fontaine-Aupart Fluorescence Correlation Spectroscopy To Study Diffusion and Reaction of Bacteriophages inside Biofilms Appl. Envir. Microbiol., April 1, 2008; 74(7): 2135 - 2143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Lu and J. J. Collins Dispersing biofilms with engineered enzymatic bacteriophage PNAS, July 3, 2007; 104(27): 11197 - 11202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ahimou, M. J. Semmens, G. Haugstad, and P. J. Novak Effect of Protein, Polysaccharide, and Oxygen Concentration Profiles on Biofilm Cohesiveness Appl. Envir. Microbiol., May 1, 2007; 73(9): 2905 - 2910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Xavier and K. R. Foster From the Cover: Cooperation and conflict in microbial biofilms PNAS, January 16, 2007; 104(3): 876 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Curtin and R. M. Donlan Using Bacteriophages To Reduce Formation of Catheter-Associated Biofilms by Staphylococcus epidermidis. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1268 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lacqua, O. Wanner, T. Colangelo, M. G. Martinotti, and P. Landini Emergence of Biofilm-Forming Subpopulations upon Exposure of Escherichia coli to Environmental Bacteriophages Appl. Envir. Microbiol., January 1, 2006; 72(1): 956 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Xavier, C. Picioreanu, S. A. Rani, M. C. M. van Loosdrecht, and P. S. Stewart Biofilm-control strategies based on enzymic disruption of the extracellular polymeric substance matrix - a modelling study Microbiology, December 1, 2005; 151(12): 3817 - 3832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kadouri and G. A. O'Toole Susceptibility of Biofilms to Bdellovibrio bacteriovorus Attack Appl. Envir. Microbiol., July 1, 2005; 71(7): 4044 - 4051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Levesque, M. Duplessis, J. Labonte, S. Labrie, C. Fremaux, D. Tremblay, and S. Moineau Genomic Organization and Molecular Analysis of Virulent Bacteriophage 2972 Infecting an Exopolysaccharide-Producing Streptococcus thermophilus Strain Appl. Envir. Microbiol., July 1, 2005; 71(7): 4057 - 4068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. W. Sutherland Biofilm exopolysaccharides: a strong and sticky framework Microbiology, January 1, 2001; 147(1): 3 - 9. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
