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The product of the yvoC(geri)gene of Bacillus subtillis is required for spore germination

Carl Robinson^1,

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield 510 ZTN, UK
lnstitut de GCnCtique et de Biologie Microbiennes, UniversitC de Lausanne, Rue CCsar-Roux 19, CH-1005 Lausanne, Switzerland

³Author for correspondence: Anne Moir. Tel: +44 114 222 4418. Fax: +44 114 272 8697.e-mail: a.moir@sheffield.ac.uk

ABSTRACT

SUMMARY: All known gerF mutations affecting Bacillus subtilis spore germination have been mapped, by a combination of recombination and complementation analysis, to yvoC (/gt)# a gene belonging to the yvoB CptsK) yvoC (/gt) yvoDEF operon. Examination of the properties of null mutants confirmed that the only gene in the operon that affects germination is poC, which encodes a homologue of known prelipoprotein diacylglyceryl transferases. As several germination proteins (GerAC, GerBC, GerKC, GerD) are predicted lipoproteins, it is not unreasonable to assume that a defect in prelipoprotein processing will affect spore germination. Two other null mutants in this chromosomal region showed a clear phenotype: the nagA gene is required for growth on N-acetylglucosamine, whereas a null mutation in yvoB (ptsK) affects colony formation from single cells.

Present address: Department of Biochemistry, University of Portsmouth, S t Michael's Building, White Swan Rd, Portsmouth PO1 2DT. UK.

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