| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Bacterial Genetics, Institute of Microbiology, Warsaw University, Nowy Swiat 67,00-046 Warsaw, Poland
2Author for correspondence: Dariusz Bartosik. Tel/Fax: +48 22 826 89 82. e-mail: bartosik@plearn.edu.
ABSTRACT
SUMMARY: The second replicator region of the native plasmid pTAVl of Paracoccus versutus has been identified thus proving the composite nature of this replicon. The minimal replicon designated pTAV320 (43 kb) was cloned and sequenced. pTAV320 encodes three putative proteins - RepA, RepB and RepC. This replicator region shows strong structural and functional similarity to mpABC-type rep1 icons found in several Agrobacterium and Rhizobiumplasmids. The origin of replication appears to be localized within the coding sequence of the repC gene. RepC was shown t o be essential for replication. RepA and RepB were necessary for stable maintenance of the plasmid, which implies a role in active partitioning. The presence of the complete sequence of pTAV320 (in its non-replicative form) could stabilize in cis pTAV202, a rninireplicon derived from the other pTAVl replicator region. Deletions introduced into the mpC gene abolished the ‘stabilizing’ activity of pTAV320, suggesting that the centromere-like sequence, necessary for partitioning, might be localized within this gene. The two replicator regions of pTAV1 (pTAV320 and pTAV202) expressed incompatibility towards the parental plasmid but were compatible in trans in P. wersutus cells. The pTAV320 replicon can be maintained in several Paracoccus, Agrobacterium, Rhizobium and Rhodohcter strains in addition t o P. versutus.
This article has been cited by other articles:
|
|
 |
|
  B. M. Gruner, S.-R. Han, H.-G. Meyer, U. Wulf, S. Bhakdi, and E. K. Siegel Characterization of a Catalase-Negative Methicillin-Resistant Staphylococcus aureus Strain J. Clin. Microbiol., August 1, 2007; 45(8): 2684 - 2685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Dziewit, M. Jazurek, L. Drewniak, J. Baj, and D. Bartosik The SXT Conjugative Element and Linear Prophage N15 Encode Toxin-Antitoxin-Stabilizing Systems Homologous to the tad-ata Module of the Paracoccus aminophilus Plasmid pAMI2 J. Bacteriol., March 1, 2007; 189(5): 1983 - 1997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stiens, S. Schneiker, M. Keller, S. Kuhn, A. Puhler, and A. Schluter Sequence Analysis of the 144-Kilobase Accessory Plasmid pSmeSM11a, Isolated from a Dominant Sinorhizobium meliloti Strain Identified during a Long-Term Field Release Experiment. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3662 - 3672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mikosa, M. Sochacka-Pietal, J. Baj, and D. Bartosik Identification of a transposable genomic island of Paracoccus pantotrophus DSM 11072 by its transposition to a novel entrapment vector pMMB2. Microbiology, April 1, 2006; 152(Pt 4): 1063 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bartosik, M. Sochacka, and J. Baj Identification and Characterization of Transposable Elements of Paracoccus pantotrophus J. Bacteriol., July 1, 2003; 185(13): 3753 - 3763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bartosik, J. Baj, M. Sochacka, E. Piechucka, and M. Wlodarczyk Molecular characterization of functional modules of plasmid pWKS1 of Paracoccus pantotrophus DSM 11072 Microbiology, September 1, 2002; 148(9): 2847 - 2856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bartosik, J. Baj, A. A. Bartosik, and M. Wlodarczyk Characterization of the replicator region of megaplasmid pTAV3 of Paracoccus versutus and search for plasmid-encoded traits Microbiology, March 1, 2002; 148(3): 871 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bartosik, M. Szymanik, and E. Wysocka Identification of the Partitioning Site within the repABC-Type Replicon of the Composite Paracoccus versutus Plasmid pTAV1 J. Bacteriol., November 1, 2001; 183(21): 6234 - 6243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. G. Chain, I. Hernandez-Lucas, B. Golding, and T. M. Finan oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region J. Bacteriol., October 1, 2000; 182(19): 5486 - 5494. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Ramírez-Romero, N. Soberón, A. Pérez-Oseguera, J. Téllez-Sosa, and M. A. Cevallos Structural Elements Required for Replication and Incompatibility of the Rhizobium etli Symbiotic Plasmid J. Bacteriol., June 1, 2000; 182(11): 3117 - 3124. [Abstract] [Full Text]
|
|
|
|
|
|
P.-L. Li and S. K. Farrand The Replicator of the Nopaline-Type Ti Plasmid pTiC58 Is a Member of the repABC Family and Is Influenced by the TraR-Dependent Quorum-Sensing Regulatory System J. Bacteriol., January 1, 2000; 182(1): 179 - 188. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
