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Microbiology 144 (1998), 3317-3325
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microbiology, Vol 144, 3317-3325, Copyright © 1998 by Society for General Microbiology

ARTICLES

Analysis of the effect exerted by extracellular pH on the maltose regulon in Escherichia coli K-12

S Alonzo, M Heyde, P Laloi and R Portalier
Centre de Genetique Moleculaire et Cellulaire, UMR CNRS 5534, Universite Claude Bernard, Villeurbanne, France.

The Escherichia coli maltose regulon consists of five operons under the control of the MalT transcriptional activator. lac operon fusions were constructed in vitro with the MalT-dependent promoter and with the malT promoter itself. beta-Galactosidase activity displayed by these fusions during growth at different external pH (pHo) revealed that growth at a pHo higher than 6 stimulates the transcription of malT- and MalT- controlled genes in the absence or presence of maltose. Using a malTp1 malTp10 promoter that is cAMP-CRP (cAMP receptor protein)-independent, it was demonstrated that CRP is essential for malT pHo regulation and that the pHo-dependent activity of malKp is a direct consequence of malT regulation. The pHo regulation displayed by a deleted but still functional malT promoter fused to lacZ demonstrates that this minimal promoter contains all the regulatory regions for establishing pHo regulation. In the absence of MIc, a repressor of malT expression, the pHo regulation of malT was still effective. It is proposed that binding of cAMP-CRP at malTp may be affected by malTp topology induced by pHo or that a pHo-dependent effector may act in concert with the cAMP-CRP complex.


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Copyright © 1998 Society for General Microbiology.