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Microbiology 144 (1998), 3343-3349; DOI  10.1099/00221287-144-12-3343
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Heterologous expression of a basic elicitin from Phytophthora cryptogea in Phytophthora infestans increases its ability to cause leaf necrosis in tobacco

Franck Panabières2, Paul R. J. Birch1, Shiela E. Unkles1, Michel Ponchet2, Isabelle Lacourt1, Paul Venard2, Harald Keller2, Valerie Allasia2, Pierre Ricci2 and James M. Duncan1

1 Fungal and Bacterial Plant Pathology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
2 Station de Botanique et de Pathologie Végétale, INRA, BP 2078, F-06606 Antibes, France

ABSTRACT

Summary: The cry-b sequence, encoding a basic elicitin (cryptogein B) from Phytophthora cryptogea, was co-transformed into Phytophthora infestans. The copy number of the cry-b sequence varied in co-transformants. Nevertheless, in all cases the alien elicitin gene was transcribed, translated and the protein secreted in vitro from such transformants. Moreover, the secreted cryptogein B from P. infestans co-transformants increased their ability to cause a hypersensitive-response-like necrosis of tobacco leaves. It was thus concluded that the transfer of a single gene encoding a basic elicitin from one Phytophthora species to another can dramatically alter the phenotypic interaction of the transformed species with tobacco.

Author for correspondence: Paul R. J. Birch. Tel: +44 1382 562731. Fax: +44 1382 562426. e-mail: pbirch@scri.sari.ac.uk


Keywords: co-transformation, elicitin gene, heterologous expression, Phytothora, tobacco necrosis







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