HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Blackall, P. J. Articles by Hampson, D. J. Search for Related Content PubMed PubMed Citation Articles by Blackall, P. J. Articles by Hampson, D. J. Agricola Articles by Blackall, P. J. Articles by Hampson, D. J.

Population structure and diversity of avian isolates of Pasteurella multocida from Australia

Narelle Fegan

Animal Research Institute, Yeerongpilly, Queensland 4105, Australia
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia

ABSTRACT

A total of 110 isolates of Pasteurella multocida from Australian poultry and reference strains for the 16 somatic serovars plus the three subspecies (gallicida, multocida, septica) were analysed to examine their population structure and diversity. The 81 field isolates examined by multilocus enzyme electrophoresis (MLEE) were diverse, being divided into 56 electrophoretic types (ETs), with the 19 reference strains in another 15 ETs. The population was clonal and somatic serotyping was not particularly useful in establishing relationships between isolates. The 71 ETs formed three distinct subclusters (A, B and C) at a genetic distance of 0.36. Biovars tended to be associated with these subclusters: A with biovars 1, 3, 4, 5 and 8 and B with biovars 2, 6, 7, 9 and 10. Ribotyping, performed on all 110 isolates using Hpall, recognized 21 ribotypes forming nine clusters (R1-R9). The isolates in ribotype cluster R1 were almost identical to those in MLEE cluster B. Using both MLEE and ribotyping, the 19 non-Australian reference strains were found to be distributed over the full diversity of the Australian isolates of P. multocida. This study has shown that a range of P. multocida clones are associated with fowl cholera in Australia and that many of the Australian isolates are similar to non-Australian reference strains. Both the MLEE results and the ribotyping data identified a previously unrecognized subset of P. multocida strains.

Pat Blackall. Tel: +61 7 33629498. Fax: +61 7 33629429. e-mail: blackap@dpi.qld.gov.au

Present address: CSIRO, Division of Food Science & Technology, PO Box 3312, Tingalpa DC 4173, Australia.

This article has been cited by other articles:

				H. Christensen, P. Kuhnert, H.-J. Busse, W. C. Frederiksen, and M. Bisgaard Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae Int J Syst Evol Microbiol, January 1, 2007; 57(1): 166 - 178. [Abstract] [Full Text] [PDF]

				H. Christensen, M. Bisgaard, O. Angen, W. Frederiksen, and J. E. Olsen Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds J. Clin. Microbiol., January 1, 2005; 43(1): 259 - 270. [Abstract] [Full Text] [PDF]

				R. L. Davies Genetic diversity among Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of the 16S rRNA gene Microbiology, December 1, 2004; 150(12): 4199 - 4210. [Abstract] [Full Text] [PDF]

				H. Christensen, O. Angen, J. E. Olsen, and M. Bisgaard Revised description and classification of atypical isolates of Pasteurella multocida from bovine lungs based on genotypic characterization to include variants previously classified as biovar 2 of Pasteurella canis and Pasteurella avium Microbiology, June 1, 2004; 150(6): 1757 - 1767. [Abstract] [Full Text] [PDF]

				A. M. Bojesen, M. Torpdahl, H. Christensen, J. E. Olsen, and M. Bisgaard Genetic Diversity of Gallibacterium anatis Isolates from Different Chicken Flocks J. Clin. Microbiol., June 1, 2003; 41(6): 2737 - 2740. [Abstract] [Full Text] [PDF]

				R. L. Davies, R. MacCorquodale, S. Baillie, and B. Caffrey Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis J. Med. Microbiol., January 1, 2003; 52(1): 59 - 67. [Abstract] [Full Text] [PDF]

				K. D. Petersen, H. Christensen, M. Bisgaard, and J. E. Olsen Genetic diversity of Pasteurella multocida fowl cholera isolates as demonstrated by ribotyping and 16S rRNA and partial atpD sequence comparisons Microbiology, October 1, 2001; 147(10): 2739 - 2748. [Abstract] [Full Text] [PDF]