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Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Instituto de Microbiologia Professor Paulo de Góes, UFRJ, Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Ilha do Fundão, 21949-970, Rio de Janeiro, RJ, Brazil
Centro de Biocicias e Biotecnologia, Universidade Estadual do Norte Fluminense, 28015-620, Campos, RJ, Brazil
Disciplina de Biologia Celular, Universidade Federal de São Paulo, 04023-062, São Paulo, SP, Brazil

ABSTRACT

The surface anionogenic groups and sialoglycoconjugate structures of Paracoccidioides brasiliensis yeast forms were analysed by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion and flow cytofluorimetry. P. brasiliensis yeast forms, particularly the budding primordia, reacted strongly with cationized ferritin. Binding assays showed that the reaction with sialic-acid-specific Limax flavus lectin (LFA) was distributed over the entire P. brasiliensis cell wall. Treatment of yeast forms with neuraminidase significantly reduced their negative surface charge and LFA labelling, which suggests that sialic acid residues are major anionogenic groups exposed on the P. brasiliensis surface. Furthermore, after neuraminidase treatment, labelling with Arachis hypogaea (peanut) agglutinin increased due to unmasking of subterminal βD-galactopyranosyl residues. The sialic acid linkages to galactose are 2,6 and 2,3 as assessed, respectively, by fungal attachment to M1/5 and M1/5 HS8 strains of influenza A virus and binding of Sambucus niger and Maackia amurensis agglutinins. The 2,6 linkage clearly predominated in both experiments. Flow cytofluorimetry analysis revealed the heterogenicity of P. brasiliensis yeast cell populations, which comprised young and mature budding yeasts. Both express binding sites to LFA and Limulus polyphemus agglutinin.

Author for correspondence: Celuta S. Alviano. Tel: +55 21 560 8344. Fax: +55 21 560 8344. e-mail: immgceu@microbio.ufrj.br

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