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Microbiology 144 (1998), 509-517; DOI  10.1099/00221287-144-2-509
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Physical and genetic map of the genome of Staphylococcus carnosus TM300

Elke Wagner1, Jirí Doskar2 and Friedrich Götz1

Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic

ABSTRACT

A genome map of Staphylococcus carnosus TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome.

Friedrich Götz. Tel: +49 7071 2974636. Fax: +49 7071 295937. e-mail: friedrich.goetz@uni-tuebingen.de

Keywords: genome mapping, Staphylococcus carnosus




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A.-M. Dudez, S. Chaillou, L. Hissler, R. Stentz, M.-C. Champomier-Verges, C.-A. Alpert, and M. Zagorec
Physical and genetic map of the Lactobacillus sakei 23K chromosome
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