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microbiology, Vol 144, 509-517, Copyright © 1998 by Society for General Microbiology

ARTICLES

Physical and genetic map of the genome of Staphylococcus carnosus TM300

E Wagner, J Doskar and F Gotz
Lehrstuhl fur Mikrobielle Genetik, Universitat Tubingen, Germany.

A genome map of Staphylococcus carnosus TM300, an important micro- organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome.


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A.-M. Dudez, S. Chaillou, L. Hissler, R. Stentz, M.-C. Champomier-Verges, C.-A. Alpert, and M. Zagorec
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