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Microbiology 144 (1998), 1013-1019
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microbiology, Vol 144, 1013-1019, Copyright © 1998 by Society for General Microbiology

ARTICLES

The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

XX Gu, R Rossau, G Jannes, R Ballard, M Laga and E Van Dyck
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium. guxiaoxiao@hotmail.com

The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.


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