HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Regamey, A. Articles by Karamata, D. Search for Related Content PubMed PubMed Citation Articles by Regamey, A. Articles by Karamata, D. Agricola Articles by Regamey, A. Articles by Karamata, D.

The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SPβ

Institut de génétique et de biologie microbiennes, Rue César-Roux 19, CH-1005 Lausanne, Switzerland

ABSTRACT

Heat shock of Bacillus subtilis CU1147, a strain lysogenic for SPβc2, a prophage with a thermosensitive repressor, results in phage induction and subsequent cell lysis. Cloning in Escherichia coli and sequencing of a DNA fragment of prophage SPβ led to the identification of blyA, the gene encoding a 367 amino acid polypetide with a molecular mass of 39.6 kDa. Purified BlyA obtained from the E. coli clone exhibited an N-acetylmuramoyl-L-alanine amidase activity. Insertional mutagenesis confirmed that the latter enzyme was associated with SPβ-phage-mediated ceil lysis. Analysis of the neighbouring sequence suggested that the two ORFs immediately downstream of blyA and belonging to the same operon encode polypeptides which may be involved in the release of the endolysin. The presence on the chromosomes of B. subtilis or related Bacillus spp. of other, similar genes, and their possible relationship, is discussed.

^*Author for correspondence: Dimitri Karamata. Tel: + 41 21 3206075. Fax: +41 21 3206078. e-mail: dimitri.karamata@igbm.unil.ch

This article has been cited by other articles:

				M. Albano, W. K. Smits, L. T. Y. Ho, B. Kraigher, I. Mandic-Mulec, O. P. Kuipers, and D. Dubnau The Rok Protein of Bacillus subtilis Represses Genes for Cell Surface and Extracellular Functions J. Bacteriol., March 15, 2005; 187(6): 2010 - 2019. [Abstract] [Full Text] [PDF]

				H. Tjalsma, H. Antelmann, J. D.H. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J.-Y. F. Dubois, H. Westers, G. Zanen, W. J. Quax, et al. Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome Microbiol. Mol. Biol. Rev., June 1, 2004; 68(2): 207 - 233. [Abstract] [Full Text] [PDF]

				H. Antelmann, H. Tjalsma, B. Voigt, S. Ohlmeier, S. Bron, J. M. van Dijl, and M. Hecker A Proteomic View on Genome-Based Signal Peptide Predictions Genome Res., September 1, 2001; 11(9): 1484 - 1502. [Abstract] [Full Text] [PDF]

				T. J. Smith, S. A. Blackman, and S. J. Foster Autolysins of Bacillus subtilis: multiple enzymes with multiple functions Microbiology, February 1, 2000; 146(2): 249 - 262. [Full Text]

				F. A. Nugroho, H. Yamamoto, Y. Kobayashi, and J. Sekiguchi Characterization of a New Sigma-K-Dependent Peptidoglycan Hydrolase Gene That Plays a Role in Bacillus subtilis Mother Cell Lysis J. Bacteriol., October 15, 1999; 181(20): 6230 - 6237. [Abstract] [Full Text]