| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Bacteriology Group Area Science Park, Padriciano 99, 34012 Trieste, Italy
Microbiology Group International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, 34012 Trieste, Italy
ABSTRACT
Transposon Tn5 genomic mutants of plant-growth-promoting Pseudomonas putida strain WCS358 have been isolated which no longer utilize ferulic and coumaric acids as sole sources of carbon and energy. Genetic studies confirmed previous biochemical data showing that ferulic acid is degraded via vanillic acid, and coumaric acid via hydroxybenzoic acid. The genes involved in these enzymic steps were cloned and characterized. Two proteins designated Fca (26.5 kDa) and Vdh (50.3 kDa) were identified as responsible for the conversion of ferulic acid to vanillic acid; the proteins are encoded by the fca and vdh genes which are organized in an operon structure in the chromosome. The Vdh protein is 69% identical at the amino acid level to the Vdh protein recently identified in Pseudomonas sp. strain HR199 and converts vanillin to vanillic acid. Homology studies revealed that the Vdh proteins exhibited significant identity to aldehyde dehydrogenases from different organisms whereas Fca belonged to the enoyl-CoA hydratase family of proteins. Two proteins, designated VanA (39.9 kDa) and VanB (34.3 kDa), encoded by two genes, vanA and vanB, are organized in an operon in the chromosome. They were found to be responsible for the demethylation of vanillic acid to protocatechuic acid. The VanA proteins showed no homology to any other known protein, while VanB belonged to the ferredoxin family of proteins. This two-component enzyme system demethylated another phenolic monomer, veratric acid, thus indicating broad specificity. Studies of the regulation of the vanAB operon demonstrated that the genes were induced by the substrate, vanillic acid; however, the strongest induction was observed when cells were grown in the presence of the product of the reaction, protocatechuic acid.
*Author for correspondence: Vittorio Venturi. Tel: +39 40 3757317. Fax: +39 40 226555. e-mail: venturi@icgeb.trieste.it
This article has been cited by other articles:
|
|
 |
|
  L. Steindler, I. Bertani, L. De Sordi, S. Schwager, L. Eberl, and V. Venturi LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants Appl. Envir. Microbiol., August 1, 2009; 75(15): 5131 - 5140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Hansen, B. L. Moller, G. R. Kock, C. M. Bunner, C. Kristensen, O. R. Jensen, F. T. Okkels, C. E. Olsen, M. S. Motawia, and J. Hansen De Novo Biosynthesis of Vanillin in Fission Yeast (Schizosaccharomyces pombe) and Baker's Yeast (Saccharomyces cerevisiae) Appl. Envir. Microbiol., May 1, 2009; 75(9): 2765 - 2774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Providenti, J. M. O'Brien, J. Ruff, A. M. Cook, and I. B. Lambert Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020 J. Bacteriol., June 1, 2006; 188(11): 3862 - 3869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Peng, E. Masai, D. Kasai, K. Miyauchi, Y. Katayama, and M. Fukuda A Second 5-Carboxyvanillate Decarboxylase Gene, ligW2, Is Important for Lignin-Related Biphenyl Catabolism in Sphingomonas paucimobilis SYK-6 Appl. Envir. Microbiol., September 1, 2005; 71(9): 5014 - 5021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Abe, E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda A Tetrahydrofolate-Dependent O-Demethylase, LigM, Is Crucial for Catabolism of Vanillate and Syringate in Sphingomonas paucimobilis SYK-6 J. Bacteriol., March 15, 2005; 187(6): 2030 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bertani and V. Venturi Regulation of the N-Acyl Homoserine Lactone-Dependent Quorum-Sensing System in Rhizosphere Pseudomonas putida WCS358 and Cross-Talk with the Stationary-Phase RpoS Sigma Factor and the Global Regulator GacA Appl. Envir. Microbiol., September 1, 2004; 70(9): 5493 - 5502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Parke and L. N. Ornston Toxicity Caused by Hydroxycinnamoyl-Coenzyme A Thioester Accumulation in Mutants of Acinetobacter sp. Strain ADP1 Appl. Envir. Microbiol., May 1, 2004; 70(5): 2974 - 2983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Masai, M. Sasaki, Y. Minakawa, T. Abe, T. Sonoki, K. Miyauchi, Y. Katayama, and M. Fukuda A Novel Tetrahydrofolate-Dependent O-Demethylase Gene Is Essential for Growth of Sphingomonas paucimobilis SYK-6 with Syringate J. Bacteriol., May 1, 2004; 186(9): 2757 - 2765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Parke and L. N. Ornston Hydroxycinnamate (hca) Catabolic Genes from Acinetobacter sp. Strain ADP1 Are Repressed by HcaR and Are Induced by Hydroxycinnamoyl-Coenzyme A Thioesters Appl. Envir. Microbiol., September 1, 2003; 69(9): 5398 - 5409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Torres, G. Porras, J. L. Garcia, and E. Diaz Regulation of the mhp Cluster Responsible for 3-(3-Hydroxyphenyl)propionic Acid Degradation in Escherichia coli J. Biol. Chem., July 18, 2003; 278(30): 27575 - 27585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Smith, V. B. Weaver, D. M. Young, and L. N. Ornston Genes for Chlorogenate and Hydroxycinnamate Catabolism (hca) Are Linked to Functionally Related Genes in the dca-pca-qui-pob-hca Chromosomal Cluster of Acinetobacter sp. Strain ADP1 Appl. Envir. Microbiol., January 1, 2003; 69(1): 524 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Masai, K. Harada, X. Peng, H. Kitayama, Y. Katayama, and M. Fukuda Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6 Appl. Envir. Microbiol., September 1, 2002; 68(9): 4416 - 4424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Diaz, A. Ferrandez, M. A. Prieto, and J. L. Garcia Biodegradation of Aromatic Compounds by Escherichia coli Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 523 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Bertani, M. Kojic, and V. Venturi Regulation of the p-hydroxybenzoic acid hydroxylase gene (pobA) in plant-growth-promoting Pseudomonas putida WCS358 Microbiology, June 1, 2001; 147(6): 1611 - 1620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Trautwein and U. Gerischer Effects Exerted by Transcriptional Regulator PcaU from Acinetobacter sp. Strain ADP1 J. Bacteriol., February 1, 2001; 183(3): 873 - 881. [Abstract] [Full Text]
|
|
|
|
|
|
L. Barthelmebs, C. Divies, and J.-F. Cavin Knockout of the p-Coumarate Decarboxylase Gene from Lactobacillus plantarum Reveals the Existence of Two Other Inducible Enzymatic Activities Involved in Phenolic Acid Metabolism Appl. Envir. Microbiol., August 1, 2000; 66(8): 3368 - 3375. [Abstract] [Full Text]
|
|
|
|
|
|
C. Civolani, P. Barghini, A. R. Roncetti, M. Ruzzi, and A. Schiesser Bioconversion of Ferulic Acid into Vanillic Acid by Means of a Vanillate-Negative Mutant of Pseudomonas fluorescens Strain BF13 Appl. Envir. Microbiol., June 1, 2000; 66(6): 2311 - 2317. [Abstract] [Full Text]
|
|
|
|
|
|
J. Overhage, H. Priefert, and A. Steinbüchel Biochemical and Genetic Analyses of Ferulic Acid Catabolism in Pseudomonas sp. Strain HR199 Appl. Envir. Microbiol., November 1, 1999; 65(11): 4837 - 4847. [Abstract] [Full Text]
|
|
|
|
|
|
J. Mampel, J. Ruff, F. Junker, and A. M. Cook The oxygenase component of the2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1 Microbiology, November 1, 1999; 145(11): 3255 - 3264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Segura, P. V. Bünz, D. A. D'Argenio, and L. N. Ornston Genetic Analysis of a Chromosomal Region Containing vanA and vanB, Genes Required for Conversion of Either Ferulate or Vanillate to Protocatechuate in Acinetobacter J. Bacteriol., June 1, 1999; 181(11): 3494 - 3504. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. D'Argenio, A. Segura, W. M. Coco, P. V. Bünz, and L. N. Ornston The Physiological Contribution of Acinetobacter PcaK, a Transport System That Acts upon Protocatechuate, Can Be Masked by the Overlapping Specificity of VanK J. Bacteriol., June 1, 1999; 181(11): 3505 - 3515. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
