| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA
Department of Oral Pathology, University of Sheffield, Sheffield S10 2TA, UK
ABSTRACT
Oral streptococci such as Streptococcus gordonii bind the abundant salivary enzyme x-amylase. This interaction may be important in dental plaque formation and metabolism, thus contributing to the initiation and progression of dental caries and periodontal disease, the two most common plaque-mediated diseases. The conjugative transposon Tn916 was used to insertionally inactivate gene(s) essential to the expression of amylase-binding components of S. gordonii Challis, and a mutant deficient in amylase-binding (Challis Tn1) was identified. While wild-type strains of S. gordonii released both 20 kDa and 82 kDa amylase-binding proteins into culture supernatants, Challis Tn1 expressed the 82 kDa but not the 20 kDa protein. The 20 kDa amylase-binding protein was isolated from culture supernatants of S. gordonii Challis by hydroxyapatite chromatography. A partially purified, functionally active 20 kDa protein was sequenced from blots, and the N-terminal sequence obtained was found to be DEP (A) TDAAT(R)NND. A novel strategy, based on the single-specific-primer polymerase chain reaction technique, enabled the gene inactivated by Tn916 to be cloned. Analysis of the resultant nucleotide sequence revealed an open reading frame of 585 bp, designated amylase-binding protein A (abpA), encoding a protein of 20 kDa (AbpA), immediately downstream from the insertion site of Tn916. This protein possessed a potential signal peptide followed by a region having identity with the N-terminal sequence of the 20 kDa amylase-binding protein. These results demonstrate the role of the 20 kDa protein in the binding of amylase to S. gordonii. Knowledge of the nature of amylase-binding proteins may provide a better understanding of the role of these proteins in the colonization of S. gordonii in the oral cavity.
*Author for correspondence: Frank A. Scannapieco. Tel: +1 716 829 2013. Fax: +1 716 829 3942. e-mail: Frank_Scannapieco@sdm.buffalo.edu
This article has been cited by other articles:
|
|
 |
|
  A. H. Nobbs, R. J. Lamont, and H. F. Jenkinson Streptococcus Adherence and Colonization Microbiol. Mol. Biol. Rev., September 1, 2009; 73(3): 407 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Davies, G. Svensater, and M. C. Herzberg Identification of novel LPXTG-linked surface proteins from Streptococcus gordonii Microbiology, June 1, 2009; 155(6): 1977 - 1988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Chaudhuri, S. Paju, E. M. Haase, M. M. Vickerman, J. M. Tanzer, and F. A. Scannapieco Amylase-Binding Protein B of Streptococcus gordonii Is an Extracellular Dipeptidyl-Peptidase Infect. Immun., October 1, 2008; 76(10): 4530 - 4537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Nobbs, Y. Zhang, A. Khammanivong, and M. C. Herzberg Streptococcus gordonii Hsa Environmentally Constrains Competitive Binding by Streptococcus sanguinis to Saliva-Coated Hydroxyapatite J. Bacteriol., April 15, 2007; 189(8): 3106 - 3114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, Y. Lei, A. Nobbs, A. Khammanivong, and M. C. Herzberg Inactivation of Streptococcus gordonii SspAB Alters Expression of Multiple Adhesin Genes Infect. Immun., June 1, 2005; 73(6): 3351 - 3357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Tanzer, L. Grant, A. Thompson, L. Li, J. D. Rogers, E. M. Haase, and F. A. Scannapieco Amylase-binding proteins A (AbpA) and B (AbpB) differentially affect colonization of rats' teeth by Streptococcus gordonii Microbiology, September 1, 2003; 149(9): 2653 - 2660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Gilmore, P. Srinivas, D. R. Akins, K. L. Hatter, and M. S. Gilmore Growth, Development, and Gene Expression in a Persistent Streptococcus gordonii Biofilm Infect. Immun., August 1, 2003; 71(8): 4759 - 4766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Grap, C. L. Munro, B. Ashtiani, and S. Bryant Oral Care Interventions in Critical Care: Frequency and Documentation Am. J. Crit. Care., March 1, 2003; 12(2): 113 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Rogers, R. J. Palmer Jr., P. E. Kolenbrander, and F. A. Scannapieco Role of Streptococcus gordonii Amylase-Binding Protein A in Adhesion to Hydroxyapatite, Starch Metabolism, and Biofilm Formation Infect. Immun., November 1, 2001; 69(11): 7046 - 7056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Rogers and F. A. Scannapieco RegG, a CcpA Homolog, Participates in Regulation of Amylase-Binding Protein A Gene (abpA) Expression in Streptococcus gordonii J. Bacteriol., June 1, 2001; 183(11): 3521 - 3525. [Abstract] [Full Text]
|
|
|
|
|
|
M. Jarosch, E. M. Egelseer, C. Huber, D. Moll, D. Mattanovich, U. B. Sleytr, and M. Sára Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms Microbiology, May 1, 2001; 147(5): 1353 - 1363. [Abstract] [Full Text]
|
|
|
|
|
|
C. Y. Loo, D. A. Corliss, and N. Ganeshkumar Streptococcus gordonii Biofilm Formation: Identification of Genes that Code for Biofilm Phenotypes J. Bacteriol., March 1, 2000; 182(5): 1374 - 1382. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. O’Sullivan, H. F. Jenkinson, and R. D. Cannon Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface Microbiology, January 1, 2000; 146(1): 41 - 48. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. E. Brown, J. D. Rogers, E. M. Haase, P. M. Zelasko, and F. A. Scannapieco Prevalence of the Amylase-Binding Protein A Gene (abpA) in Oral Streptococci J. Clin. Microbiol., December 1, 1999; 37(12): 4081 - 4085. [Abstract] [Full Text]
|
|
|
|
|
|
E. M. Haase, J. L. Zmuda, and F. A. Scannapieco Identification and Molecular Analysis of Rough-Colony-Specific Outer Membrane Proteins of Actinobacillus actinomycetemcomitans Infect. Immun., June 1, 1999; 67(6): 2901 - 2908. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
