| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institut für Mikrobiologie der Georg-August-Universität, Grisebachstrasse 8, D-37077 Göttingen, Germany
ABSTRACT
The telomers of several linear plasmids of Rhodococcus opacus (formerly Nocardia opaca) were studied. The plasmids pHG201, pHG204 and pHG205 carry proteins bound to their ends, as shown by gel retardation experiments. A sequence hybridizing with the terminal sequence of pHG207, a recombinant linear plasmid consisting of the left part of pHG204 and the right part of pHG205, which was analysed in a previous study by the authors, could be detected in all linear plasmids of the wild-type R. opacus strains MR11 and MR22. However, only pHG204 and pHG206 carry terminal inverted repeats (TIRs) like pHG207. Cloning and sequencing of the terminal fragment of pHG204 revealed a nearly perfect TIR of 1016 bp. In contrast, the termini of pHG201 and pHG205 share little homology. Sequence analysis of the two end fragments of pHG201 revealed a similarity of only 65% within the terminal 34/32 bp and a perfect TIR of only 3 bp. The results support the assumption that long TIRs are not absolutely necessary for replication and maintenance of linear plasmids.
*Author for correspondence: Hans G. Schlegel. Tel: + 49 551 39 2364, Fax: +49 551 39 3793, e-mail: hschleg1@gwdg.de
This article has been cited by other articles:
|
|
 |
|
  M. Ventura, C. Canchaya, A. Tauch, G. Chandra, G. F. Fitzgerald, K. F. Chater, and D. van Sinderen Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum Microbiol. Mol. Biol. Rev., September 1, 2007; 71(3): 495 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Parschat, J. Overhage, A. W. Strittmatter, A. Henne, G. Gottschalk, and S. Fetzner Complete Nucleotide Sequence of the 113-Kilobase Linear Catabolic Plasmid pAL1 of Arthrobacter nitroguajacolicus Ru61a and Transcriptional Analysis of Genes Involved in Quinaldine Degradation J. Bacteriol., May 15, 2007; 189(10): 3855 - 3867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Warren, W. W. L. Hsiao, H. Kudo, M. Myhre, M. Dosanjh, A. Petrescu, H. Kobayashi, S. Shimizu, K. Miyauchi, E. Masai, et al. Functional Characterization of a Catabolic Plasmid from Polychlorinated- Biphenyl-Degrading Rhodococcus sp. Strain RHA1 J. Bacteriol., November 15, 2004; 186(22): 7783 - 7795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamasaki and H. Kinashi Two Chimeric Chromosomes of Streptomyces coelicolor A3(2) Generated by Single Crossover of the Wild-Type Chromosome and Linear Plasmid SCP1 J. Bacteriol., October 1, 2004; 186(19): 6553 - 6559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Konig, D. Eulberg, J. Groning, S. Lakner, V. Seibert, S. R. Kaschabek, and M. Schlomann A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP Microbiology, September 1, 2004; 150(9): 3075 - 3087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stecker, A. Johann, C. Herzberg, B. Averhoff, and G. Gottschalk Complete Nucleotide Sequence and Genetic Organization of the 210-Kilobase Linear Plasmid of Rhodococcus erythropolis BD2 J. Bacteriol., September 1, 2003; 185(17): 5269 - 5274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Goshi, T. Uchida, A. Lezhava, M. Yamasaki, K. Hiratsu, H. Shinkawa, and H. Kinashi Cloning and Analysis of the Telomere and Terminal Inverted Repeat of the Linear Chromosome of Streptomyces griseus J. Bacteriol., June 15, 2002; 184(12): 3411 - 3415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shimizu, H. Kobayashi, E. Masai, and M. Fukuda Characterization of the 450-kb Linear Plasmid in a Polychlorinated Biphenyl Degrader, Rhodococcus sp. Strain RHA1 Appl. Envir. Microbiol., May 1, 2001; 67(5): 2021 - 2028. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
