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Microbiology 144 (1998), 2049-2061; DOI  10.1099/00221287-144-8-2049
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The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116

Ben N. Fry1,{dagger}, Victoria Korolik2, Janna A ten Brinke1, Maarten T. T Pennings2, Robert Zalm1, Bart J. J Teunis1, Peter J. Coloe2 and Bernard A. M van der Zeijst1,*,1,{ddagger}

Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
Department of Applied Biology, RMIT GPO Box 2476V, Melbourne 3001, Australia

ABSTRACT

Most Campylobacter jejuni strains express lipo-oligosaccharides. Some strains also express lipopolysaccharides (LPS), with O-antigen-like carbohydrate repeats. C. jejuni 81116 expresses an LPS containing both lipo-oligosaccharides and O-antigen-like repeats, but nothing is known about the structure or sugar composition of these LPS species. A cosmid library of the genome of C. jejuni 81116 was constructed and probed with Campylobacter hyoilei genes involved in LPS synthesis. Five cosmids hybridized with the probe and two of these expressed C. jejuni 81116 LPS in Escherichia coli. By subcloning, a 16 kb DNA region was identified which contains the genetic information required to express C. jejuni LPS. DNA sequence analysis revealed 11 ORFs homologous to genes involved in LPS synthesis of other bacteria. They consisted of three homologues of sugar biosynthesis genes, two homologues of transport genes and six homologues of sugar transferases.

*Author for correspondence: Bernard A. M. van der Zeijst. Tel: +31 30 274 41 64. Fax: +31 30 274 44 39. e-mail: ben.van.der.zeijst@rivm.nl

Keywords: Campylobacter jejuni, rfb gene cluster, lipo-oligosaccharide (LOS), lipo-polysaccharide (LPS), O-antigen

{ddagger} Present address: National Institute of Public Health and the Environment, Vaccine Division, PO Box 1, 3720 Bilthoven, The Netherlands.

{dagger} Present address: Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, 124 LaTrobe St, Melbourne, VIC 3000, Australia.




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