| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
ABSTRACT
Although some Campylobacter species are agents of gastroenteritis and periodontal disease in humans, little is known of the variety of campylobacters in the gastrointestinal tract of healthy individuals. This paper provides evidence for the existence of a previously undescribed, uncultivated Campylobacter species that may be a commensal in the healthy human gut. Saliva and faeces from 20 healthy individuals were examined by PCR assays specific for nine species of campylobacter (C. sputorum, C. concisus, C. upsaliensis, C. helveticus, C. Iari, C. fetus, C. hyointestinalis, C. jejuni and C. coli) and for the genus as a whole. Genus-specific amplicons were produced from 19 of 20 saliva samples and from 18 of 20 faecal samples. C. concisuss species-specific amplicons were produced from 19 of 20 saliva samples and 3 of 20 faecal samples. The faecal samples were all PCR-negative for other Campylobacter species. Three unidentified 16S rRNA Campylobacter genus-specific amplicons of faecal origin were sequenced. Phylogenetic analysis showed that these sequences were 99% similar, and clustered within the genus as a novel group which was termed HS (HS = healthy subject). A PCR primer pair specific for the HS group was designed from the sequence data and used to reexamine the original samples. Although it was not possible to culture the organism from faeces, specific PCR assay detected it in 10 of the 20 faecal samples, but not in any corresponding saliva samples. The authors propose that the source of the amplicons is a previously undescribed and so far uncultivated species, which they term ‘Candidatus Campylobacter hominis’.
*Author for correspondence: John Stanley. Tel: +44 181 200 4400. Fax: +44 181 200 1569. e-mail: ajlawson@hgmp.mrc.ac.uk
This article has been cited by other articles:
|
|
 |
|
  R. Aabenhus, S. L. W. On, B. L. Siemer, H. Permin, and L. P. Andersen Delineation of Campylobacter concisus Genomospecies by Amplified Fragment Length Polymorphism Analysis and Correlation of Results with Clinical Data J. Clin. Microbiol., October 1, 2005; 43(10): 5091 - 5096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Workman, G. E. Mathison, and M. C. Lavoie Pet Dogs and Chicken Meat as Reservoirs of Campylobacter spp. in Barbados J. Clin. Microbiol., June 1, 2005; 43(6): 2642 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maher, C. Finnegan, E. Collins, B. Ward, C. Carroll, and M. Cormican Evaluation of Culture Methods and a DNA Probe-Based PCR Assay for Detection of Campylobacter Species in Clinical Specimens of Feces J. Clin. Microbiol., July 1, 2003; 41(7): 2980 - 2986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S P Kulkarni, S Lever, J M J Logan, A J Lawson, J Stanley, and M S Shafi Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods J. Clin. Pathol., October 1, 2002; 55(10): 749 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Engberg, S. L. W. On, C. S. Harrington, and P. Gerner-Smidt Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in Human Fecal Samples as Estimated by a Reevaluation of Isolation Methods for Campylobacters J. Clin. Microbiol., January 1, 2000; 38(1): 286 - 291. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Lawson, J. M. J. Logan, G. L. O'neill, M. Desai, and J. Stanley Large-Scale Survey of Campylobacter Species in Human Gastroenteritis by PCR and PCR-Enzyme-Linked Immunosorbent Assay J. Clin. Microbiol., December 1, 1999; 37(12): 3860 - 3864. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
