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Microbiology 144 (1998), 2103-2111; DOI  10.1099/00221287-144-8-2103
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Transcription of the trp operon in Lactococcus lactis is controlled by antitermination in the leader region

HéléeGne Frenkiel1, Jacek Bardowski1,{dagger}, S. Dusko Ehrlich1 and Alain Chopin1,*

Laboratoire de Génètique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France

ABSTRACT

The regulatory functions of the leader region preceding the Lactococcus lactis trp operon have been studied by mutagenesis analysis. This leader presents striking similarity to ‘T-box’ leaders found upstream of many Gram-positive aminoacyl-tRNA synthetase genes and some amino acid biosynthesis operons, which are controlled by antitermination through interaction of the leader transcript with cognate uncharged tRNA. A region of the L. lactis leader transcript also contains a series of (G/U)AG repeats which, in Bacillus, are involved in the binding of the trp RNA-binding protein (TRAP) which controls trp transcription. A screen was developed for the isolation of regulatory mutants affected in the leader region. All spontaneous mutants contained deletions; point mutations were only obtained after UV-induced mutagenesis. All mutations affected the putative transcription terminator upstream of the trp operon, demonstrating that trp is indeed controlled by transcription antitermination.

*Author for correspondence: Alain Chopin. Tel: +33 1 34 65 25 30. Fax: +33 1 34 65 25 21. e-mail: achopin@biotec.jouy.inra.fr

Keywords: Lactococcus lactis, antitermination, tryptophan biosynthesis, trp operon, transcription terminator

{dagger} Present address: IBB-PAN, Zaklad Biochemii Drobnoustrojow, UI. Pawinskiego 5A, 02-106 Warszawa, Poland.




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