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Physical map of the chromosome of Aeromonas salmonicida and genomic comparisons between Aeromonas strains

Elizabeth Umelo^1,,*

Trevor J. Trust^1,

Department of Biochemistry and Microbiology and Canadian Bacterial Diseases Network, University of Victoria, Victoria, BC, V8W 3P6 Canada

ABSTRACT

I-Ceul and Pmel physical maps of the Aeromonas salmonicida A449 chromosome were constructed using PFGE. The circular chromosome of A. salmonicida A449 was estimated to be 4658.30 kb. The approximate location of several genes, including those encoding proteins implicated in virulence, were identified. The map showed that the known virulence-factor-encoding genes were not clustered. The I-Ceul genomic digestion fingerprints of several typical and atypical strains of A. salmonicida were compared. The results confirmed the homogeneity of typical strains, which provided further support for the clonality of the population structure of this group. Extensive diversity was observed in the I-Ceul digestion fingerprint of atypical strains, although a clonality was observed in the strains isolated from diseased goldfish. The results suggest that comparison of I-Ceul digestion fingerprints could be used as a powerful taxonomic tool to subdivide the atypical strains and also help clarify some of the current confusion associated with the taxonomy of the genus Aeromonas.

^*Author for correspondence: Elizabeth Umelo. Tel: +1 604 822 4417. Fax: +1 604 822 6041. e-mail: umelo@interchange.ubc.ca

Present address: Astra Research Center Boston Inc., 128 Sidney St, Cambridge, MA 01770, USA.

Present address: Department of Microbiology and Immunology, 300-6174 University Boulevard, University of British Columbia, Vancouver, BC, V6T 1Z3 Canada.

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