HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lugones, L. G. Articles by Wessels, J. G. H Search for Related Content PubMed PubMed Citation Articles by Lugones, L. G. Articles by Wessels, J. G. H Agricola Articles by Lugones, L. G. Articles by Wessels, J. G. H

A hydrophobin (ABH3) specifically secreted by vegetatively growing hyphae of Agaricus bisporus (common white button mushroom)

Molecular Plant Biology Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

ABSTRACT

Aerial mycelium and hyphal strands of Agaricus bisporus, strain U1, exhibited a rodlet pattern at their surfaces characteristic for assembled class I hydrophobins. An SDS-insoluble/trifluoroacetic-acid-soluble fraction from strands was found to contain one abundant protein with an apparent molecular mass on gel of 19 kDa. Two sequences for this protein (ABH3), typical of class I hydrophobins, could be deduced by sequencing cDNA clones obtained by RT-PCR. The two forms of the protein could be assigned to different alleles present in the two homokaryons that constitute the heterokaryotic U1 strain. ABH3 displays all the in vitro properties of a typical class I hydrophobin such as SC3 from Schizophyllum commune but is not glycosylated or otherwise post-translationally modified because the molecular mass values deduced from the amino acid sequence (9228 and 9271 Da) and derived from mass spectrometry were in good agreement. The ABH3 transcript was found to be present in the vegetative mycelium of both primary and secondary mycelium but not in the fruiting bodies, whereas the reverse was found for the ABH1 hydrophobin. Using an S. commune mutant with a disrupted SC3 gene it was found that ABH3 can substitute for SC3 in inducing formation of aerial hyphae, suggesting a role of ABH3 in the emergence of aerial hyphae and strands in A. bisporus.

^*Author for correspondence: Luis G. Lugones. Tel: +31 50 3632322. Fax: +31 50 3632272. e-mail: L.G.Lugones@biol.rug.nl

This article has been cited by other articles:

				M. Mikus, L. Hatvani, T. Neuhof, M. Komon-Zelazowska, R. Dieckmann, T. Schwecke, I. S. Druzhinina, H. von Dohren, and C. P. Kubicek Differential Regulation and Posttranslational Processing of the Class II Hydrophobin Genes from the Biocontrol Fungus Hypocrea atroviridis Appl. Envir. Microbiol., May 15, 2009; 75(10): 3222 - 3229. [Abstract] [Full Text] [PDF]

				M. Karlsson, J. Stenlid, and A. Olson Two hydrophobin genes from the conifer pathogen Heterobasidion annosum are expressed in aerial hyphae Mycologia, March 1, 2007; 99(2): 227 - 231. [Abstract] [Full Text] [PDF]

				M. M. Penas, J. Aranguren, L. Ramirez, and A. G. Pisabarro Structure of gene coding for the fruit body-specific hydrophobin Fbh1 of the edible basidiomycete Pleurotus ostreatus Mycologia, January 1, 2004; 96(1): 75 - 82. [Abstract] [Full Text] [PDF]

				M. M. Penas, B. Rust, L. M. Larraya, L. Ramirez, and A. G. Pisabarro Differentially Regulated, Vegetative-Mycelium-Specific Hydrophobins of the Edible Basidiomycete Pleurotus ostreatus Appl. Envir. Microbiol., August 1, 2002; 68(8): 3891 - 3898. [Abstract] [Full Text] [PDF]

				K. Scholtmeijer, M. I. Janssen, B. Gerssen, M. L. de Vocht, B. M. van Leeuwen, T. G. van Kooten, H. A. B. Wosten, and J. G. H. Wessels Surface Modifications Created by Using Engineered Hydrophobins Appl. Envir. Microbiol., March 1, 2002; 68(3): 1367 - 1373. [Abstract] [Full Text] [PDF]

				A. Mankel, K. Krause, and E. Kothe Identification of a Hydrophobin Gene That is Developmentally Regulated in the Ectomycorrhizal Fungus Tricholoma terreum Appl. Envir. Microbiol., March 1, 2002; 68(3): 1408 - 1413. [Abstract] [Full Text] [PDF]

				H. A. B. Wösten and J. M. Willey Surface-active proteins enable microbial aerial hyphae to grow into the air Microbiology, April 1, 2000; 146(4): 767 - 773. [Full Text]

				M. L. de Vocht, I. Reviakine, H. A. B. Wosten, A. Brisson, J. G. H. Wessels, and G. T. Robillard Structural and Functional Role of the Disulfide Bridges in the Hydrophobin SC3 J. Biol. Chem., September 8, 2000; 275(37): 28428 - 28432. [Abstract] [Full Text] [PDF]