| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Mikrobiologisches Institut, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland
Institute of Food Research, Department of Food Macromolecular Science, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK
ABSTRACT
A 5 kb region upstream of katA at 82°G on the Bacillus subtilis chromosome contains five ORFs organized in an operon-like structure. Based on sequence similarity, three of the ORFs are likely to encode an ABC transport system (ssuBAC) and another to encode a monooxygenase (ssuD). The deduced amino acid sequence of the last ORF (ygaN) shows no similarity to any known protein. B. subtilis can utilize a range of aliphatic sulfonates such as alkanesulfonates, taurine, isethionate and sulfoacetate as a source of sulfur, but not when ssuA and ssuC are disrupted by insertion of a neomycin-resistance gene. Utilization of aliphatic sulfonates was not affected in a strain lacking 3'-phosphoadenosine 5'-phosphosulfate (PAPS) sulfotransferase, indicating that sulfate is not an intermediate in the assimilation of sulfonate-sulfur. Sulfate or cysteine prevented expression of β-galactosidase from a transcriptional ssuD::IacZ fusion. It is proposed that ssuBACD encode a system for ATP-dependent transport of alkanesulfonates and an oxygenase required for their desulfonation.
*Author for correspondence: Jan R. van der Ploeg. Tel: +41 1 6323332. Fax: +41 1 6321148.
This article has been cited by other articles:
|
|
 |
|
  S. Tsukada, T. Aono, N. Akiba, K.-B. Lee, C.-T. Liu, H. Toyazaki, and H. Oyaizu Comparative Genome-Wide Transcriptional Profiling of Azorhizobium caulinodans ORS571 Grown under Free-Living and Symbiotic Conditions Appl. Envir. Microbiol., August 1, 2009; 75(15): 5037 - 5046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Kim, T. Hisano, K. Takeda, W. Iwasaki, A. Ebihara, and K. Miki Crystal Structure of the Oxygenase Component (HpaB) of the 4-Hydroxyphenylacetate 3-Monooxygenase from Thermus thermophilus HB8 J. Biol. Chem., November 9, 2007; 282(45): 33107 - 33117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Oh, B. O. Palsson, S. M. Park, C. H. Schilling, and R. Mahadevan Genome-scale Reconstruction of Metabolic Network in Bacillus subtilis Based on High-throughput Phenotyping and Gene Essentiality Data J. Biol. Chem., September 28, 2007; 282(39): 28791 - 28799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Iwanicka-Nowicka, A. Zielak, A. M. Cook, M. S. Thomas, and M. M. Hryniewicz Regulation of Sulfur Assimilation Pathways in Burkholderia cenocepacia: Identification of Transcription Factors CysB and SsuR and Their Role in Control of Target Genes J. Bacteriol., March 1, 2007; 189(5): 1675 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Y. Choi, D. Reyes, M. Leelakriangsak, and P. Zuber The Global Regulator Spx Functions in the Control of Organosulfur Metabolism in Bacillus subtilis. J. Bacteriol., August 1, 2006; 188(16): 5741 - 5751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Weir, T. D. Sutherland, I. Horne, R. J. Russell, and J. G. Oakeshott A Single Monooxygenase, Ese, Is Involved in the Metabolism of the Organochlorides Endosulfan and Endosulfate in an Arthrobacter sp. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3524 - 3530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Even, P. Burguiere, S. Auger, O. Soutourina, A. Danchin, and I. Martin-Verstraete Global Control of Cysteine Metabolism by CymR in Bacillus subtilis J. Bacteriol., March 15, 2006; 188(6): 2184 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jamshad, P. De Marco, C. C. Pacheco, T. Hanczar, and J. C. Murrell Identification, Mutagenesis, and Transcriptional Analysis of the Methanesulfonate Transport Operon of Methylosulfonomonas methylovora Appl. Envir. Microbiol., January 1, 2006; 72(1): 276 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Albanesi, M. C. Mansilla, G. E. Schujman, and D. de Mendoza Bacillus subtilis Cysteine Synthetase Is a Global Regulator of the Expression of Genes Involved in Sulfur Assimilation J. Bacteriol., November 15, 2005; 187(22): 7631 - 7638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Koch, C. Ruckert, D. A. Rey, A. Mix, A. Puhler, and J. Kalinowski Role of the ssu and seu Genes of Corynebacterium glutamicum ATCC 13032 in Utilization of Sulfonates and Sulfonate Esters as Sulfur Sources Appl. Envir. Microbiol., October 1, 2005; 71(10): 6104 - 6114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Burguiere, J. Fert, I. Guillouard, S. Auger, A. Danchin, and I. Martin-Verstraete Regulation of the Bacillus subtilis ytmI Operon, Involved in Sulfur Metabolism J. Bacteriol., September 1, 2005; 187(17): 6019 - 6030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Erwin, S. Nakano, and P. Zuber Sulfate-Dependent Repression of Genes That Function in Organosulfur Metabolism in Bacillus subtilis Requires Spx J. Bacteriol., June 15, 2005; 187(12): 4042 - 4049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Burguiere, S. Auger, M.-F. Hullo, A. Danchin, and I. Martin-Verstraete Three Different Systems Participate in L-Cystine Uptake in Bacillus subtilis J. Bacteriol., August 1, 2004; 186(15): 4875 - 4884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Lithgow, E. J. Hayhurst, G. Cohen, Y. Aharonowitz, and S. J. Foster Role of a Cysteine Synthase in Staphylococcus aureus J. Bacteriol., March 15, 2004; 186(6): 1579 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Minami, H. Suzuki, and H. Kumagai {gamma}-Glutamyltranspeptidase, but Not YwrD, Is Important in Utilization of Extracellular Glutathione as a Sulfur Source in Bacillus subtilis J. Bacteriol., February 15, 2004; 186(4): 1213 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mostertz, C. Scharf, M. Hecker, and G. Homuth Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress Microbiology, February 1, 2004; 150(2): 497 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Endoh, H. Habe, T. Yoshida, H. Nojiri, and T. Omori A CysB-regulated and {sigma}54-dependent regulator, SfnR, is essential for dimethyl sulfone metabolism of Pseudomonas putida strain DS1 Microbiology, April 1, 2003; 149(4): 991 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Schleheck, M. Lechner, R. Schonenberger, M. J.-F. Suter, and A. M. Cook Desulfonation and Degradation of the Disulfodiphenylethercarboxylates from Linear Alkyldiphenyletherdisulfonate Surfactants Appl. Envir. Microbiol., February 1, 2003; 69(2): 938 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Guillouard, S. Auger, M.-F. Hullo, F. Chetouani, A. Danchin, and I. Martin-Verstraete Identification of Bacillus subtilis CysL, a Regulator of the cysJI Operon, Which Encodes Sulfite Reductase J. Bacteriol., September 1, 2002; 184(17): 4681 - 4689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Baxter, J. Scanlan, P. De Marco, A. P. Wood, and J. C. Murrell Duplicate Copies of Genes Encoding Methanesulfonate Monooxygenase in Marinosulfonomonas methylotropha Strain TR3 and Detection of Methanesulfonate Utilizers in the Environment Appl. Envir. Microbiol., January 1, 2002; 68(1): 289 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Coppee, S. Auger, E. Turlin, A. Sekowska, J.-P. Le Caer, V. Labas, V. Vagner, A. Danchin, and I. Martin-Verstraete Sulfur-limitation-regulated proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study Microbiology, June 1, 2001; 147(6): 1631 - 1640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Mansilla, D. Albanesi, and D. de Mendoza Transcriptional Control of the Sulfur-Regulated cysH Operon, Containing Genes Involved in L-Cysteine Biosynthesis in Bacillus subtilis J. Bacteriol., October 15, 2000; 182(20): 5885 - 5892. [Abstract] [Full Text]
|
|
|
|
|
|
E. Eichhorn, J. R. van der Ploeg, and T. Leisinger Deletion Analysis of the Escherichia coli Taurine and Alkanesulfonate Transport Systems J. Bacteriol., May 15, 2000; 182(10): 2687 - 2695. [Abstract] [Full Text]
|
|
|
|
|
|
J. R. van der Ploeg, R. Iwanicka-Nowicka, T. Bykowski, M. M. Hryniewicz, and T. Leisinger The Escherichia coli ssuEADCB Gene Cluster Is Required for the Utilization of Sulfur from Aliphatic Sulfonates and Is Regulated by the Transcriptional Activator Cbl J. Biol. Chem., October 8, 1999; 274(41): 29358 - 29365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Eichhorn, J. R. van der Ploeg, and T. Leisinger Characterization of a Two-component Alkanesulfonate Monooxygenase from Escherichia coli J. Biol. Chem., September 17, 1999; 274(38): 26639 - 26646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Kertesz, K. Schmidt-Larbig, and T. Wüest A Novel Reduced Flavin Mononucleotide-Dependent Methanesulfonate Sulfonatase Encoded by the Sulfur-Regulated msu Operon of Pseudomonas aeruginosa J. Bacteriol., March 1, 1999; 181(5): 1464 - 1473. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
