| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Environmental Systems Engineering, Nagaoka University of Technology, Kamitomioka 1603-1, Nagaoka, Niigata 940-2188, Japan
National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan
ABSTRACT
The microbial diversity of two types of methanogenic granular sludge, mesophilic (35 °C) and thermophilic (55 °C), which had been treating sucrose/propionate/acetate-based artificial wastewater were compared. 16S rDNA clone libraries were constructed by PCR with a prokaryote-specific primer set, and partial sequencing of the clonal 16S rDNAs was conducted for phylogenetic analysis. Of 115 mesophilic granule and 110 thermophilic granule clones sequenced, 19 and 22%, respectively, were phylogenetically affiliated with the domain Archaea, and the remainder in each case were assigned to the domain Bacteria. Within the domain Archaea, the 16S rDNA clones in both libraries showed relatively close relationships with those of methanogens. Within the Bacteria, a major group represented in the mesophilic clone library was the delta subclass of the Proteobacteria (27%), in which high degrees of relatedness were observed between the clonal 16S rDNA sequences and those of previously identified syntrophic bacteria and sulfate-reducing bacteria. In contrast, in the thermophilic clone library, the Thermodesulfovibrio group (19%), the green non-sulfur bacteria (18%) and the low G+C subclass of the Gram-positive bacteria (18%) were predominant. A significant difference between the two libraries was that no clone affiliated with the Proteobacteria was detected in the thermophilic clone library, whereas the Proteobacteria was the most predominant group in the mesophilic clones. Thirty-six and 24 different sequences were found in the mesophilic and thermophilic clones, respectively, suggesting that the microbial diversity of the thermophilic granule was lower than that of the mesophilic granule.
*Author for correspondence: Yuji Sekiguchi. Tel: +81 258 47 1611 ext. 6313. Fax: +81 258 47 9600.
This article has been cited by other articles:
|
|
 |
|
  S. Sakai, H. Imachi, Y. Sekiguchi, I-C. Tseng, A. Ohashi, H. Harada, and Y. Kamagata Cultivation of Methanogens under Low-Hydrogen Conditions by Using the Coculture Method Appl. Envir. Microbiol., July 15, 2009; 75(14): 4892 - 4896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sekiguchi, M. Muramatsu, H. Imachi, T. Narihiro, A. Ohashi, H. Harada, S. Hanada, and Y. Kamagata Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio Int J Syst Evol Microbiol, November 1, 2008; 58(11): 2541 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sakai, H. Imachi, S. Hanada, A. Ohashi, H. Harada, and Y. Kamagata Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov. Int J Syst Evol Microbiol, April 1, 2008; 58(4): 929 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Qiu, S. Hanada, A. Ohashi, H. Harada, Y. Kamagata, and Y. Sekiguchi Syntrophorhabdus aromaticivorans gen. nov., sp. nov., the First Cultured Anaerobe Capable of Degrading Phenol to Acetate in Obligate Syntrophic Associations with a Hydrogenotrophic Methanogen Appl. Envir. Microbiol., April 1, 2008; 74(7): 2051 - 2058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Imachi, S. Sakai, Y. Sekiguchi, S. Hanada, Y. Kamagata, A. Ohashi, and H. Harada Methanolinea tarda gen. nov., sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge Int J Syst Evol Microbiol, January 1, 2008; 58(1): 294 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Satoh, Y. Miura, I. Tsushima, and S. Okabe Layered Structure of Bacterial and Archaeal Communities and Their In Situ Activities in Anaerobic Granules Appl. Envir. Microbiol., November 15, 2007; 73(22): 7300 - 7307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, H. Imachi, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata, and Y. Sekiguchi Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2299 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Imachi, S. Sakai, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata, and Y. Sekiguchi Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic, propionate-oxidizing bacterium Int J Syst Evol Microbiol, July 1, 2007; 57(7): 1487 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Meyer and J. Kuever Phylogeny of the alpha and beta subunits of the dissimilatory adenosine-5'-phosphosulfate (APS) reductase from sulfate-reducing prokaryotes - origin and evolution of the dissimilatory sulfate-reduction pathway Microbiology, July 1, 2007; 153(7): 2026 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sakai, H. Imachi, Y. Sekiguchi, A. Ohashi, H. Harada, and Y. Kamagata Isolation of Key Methanogens for Global Methane Emission from Rice Paddy Fields: a Novel Isolate Affiliated with the Clone Cluster Rice Cluster I Appl. Envir. Microbiol., July 1, 2007; 73(13): 4326 - 4331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hatamoto, H. Imachi, A. Ohashi, and H. Harada Identification and Cultivation of Anaerobic, Syntrophic Long-Chain Fatty Acid-Degrading Microbes from Mesophilic and Thermophilic Methanogenic Sludges Appl. Envir. Microbiol., February 15, 2007; 73(4): 1332 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ben-Dov, O. H. Shapiro, N. Siboni, and A. Kushmaro Advantage of Using Inosine at the 3' Termini of 16S rRNA Gene Universal Primers for the Study of Microbial Diversity Appl. Envir. Microbiol., November 1, 2006; 72(11): 6902 - 6906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, T. Terada, Y. Sekiguchi, N. Shinzato, X.-Y. Meng, M. Enoki, and Y. Kamagata Application of Pseudomurein Endoisopeptidase to Fluorescence In Situ Hybridization of Methanogens within the Family Methanobacteriaceae Appl. Envir. Microbiol., November 1, 2006; 72(11): 6907 - 6913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Imachi, Y. Sekiguchi, Y. Kamagata, A. Loy, Y.-L. Qiu, P. Hugenholtz, N. Kimura, M. Wagner, A. Ohashi, and H. Harada Non-sulfate-reducing, syntrophic bacteria affiliated with desulfotomaculum cluster I are widely distributed in methanogenic environments. Appl. Envir. Microbiol., March 1, 2006; 72(3): 2080 - 2091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ishii, T. Kosaka, K. Hori, Y. Hotta, and K. Watanabe Coaggregation Facilitates Interspecies Hydrogen Transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus Appl. Envir. Microbiol., December 1, 2005; 71(12): 7838 - 7845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, Y. Sekiguchi, H. Imachi, Y. Kamagata, A. Ohashi, and H. Harada Diversity, Localization, and Physiological Properties of Filamentous Microbes Belonging to Chloroflexi Subphylum I in Mesophilic and Thermophilic Methanogenic Sludge Granules Appl. Envir. Microbiol., November 1, 2005; 71(11): 7493 - 7503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tamaki, Y. Sekiguchi, S. Hanada, K. Nakamura, N. Nomura, M. Matsumura, and Y. Kamagata Comparative Analysis of Bacterial Diversity in Freshwater Sediment of a Shallow Eutrophic Lake by Molecular and Improved Cultivation-Based Techniques Appl. Envir. Microbiol., April 1, 2005; 71(4): 2162 - 2169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Karakashev, D. J. Batstone, and I. Angelidaki Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors Appl. Envir. Microbiol., January 1, 2005; 71(1): 331 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nocker, J. E. Lepo, and R. A. Snyder Influence of an Oyster Reef on Development of the Microbial Heterotrophic Community of an Estuarine Biofilm Appl. Envir. Microbiol., November 1, 2004; 70(11): 6834 - 6845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-L. Chen, H. Macarie, I. Ramirez, A. Olmos, S. L. Ong, O. Monroy, and W.-T. Liu Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalate Microbiology, October 1, 2004; 150(10): 3429 - 3440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Koizumi, H. Kojima, and M. Fukui Dominant Microbial Composition and Its Vertical Distribution in Saline Meromictic Lake Kaiike (Japan) as Revealed by Quantitative Oligonucleotide Probe Membrane Hybridization Appl. Envir. Microbiol., August 1, 2004; 70(8): 4930 - 4940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Uyeno, Y. Sekiguchi, A. Sunaga, H. Yoshida, and Y. Kamagata Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms Appl. Envir. Microbiol., June 1, 2004; 70(6): 3650 - 3663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Elshahed, F. Z. Najar, B. A. Roe, A. Oren, T. A. Dewers, and L. R. Krumholz Survey of Archaeal Diversity Reveals an Abundance of Halophilic Archaea in a Low-Salt, Sulfide- and Sulfur-Rich Spring Appl. Envir. Microbiol., April 1, 2004; 70(4): 2230 - 2239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-D. G. Wright, A. J. Williams, B. Winder, C. T. Christophersen, S. L. Rodgers, and K. D. Smith Molecular Diversity of Rumen Methanogens from Sheep in Western Australia Appl. Envir. Microbiol., March 1, 2004; 70(3): 1263 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Qiu, Y. Sekiguchi, H. Imachi, Y. Kamagata, I-C. Tseng, S.-S. Cheng, A. Ohashi, and H. Harada Identification and Isolation of Anaerobic, Syntrophic Phthalate Isomer-Degrading Microbes from Methanogenic Sludges Treating Wastewater from Terephthalate Manufacturing Appl. Envir. Microbiol., March 1, 2004; 70(3): 1617 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sekiguchi, T. Yamada, S. Hanada, A. Ohashi, H. Harada, and Y. Kamagata Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1843 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sekiguchi, H. Takahashi, Y. Kamagata, A. Ohashi, and H. Harada In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I Appl. Envir. Microbiol., December 1, 2001; 67(12): 5740 - 5749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Wu, W.-T. Liu, I-C. Tseng, and S.-S. Cheng Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system Microbiology, February 1, 2001; 147(2): 373 - 382. [Abstract] [Full Text]
|
|
|
|
|
|
T. M. LaPara, C. H. Nakatsu, L. Pantea, and J. E. Alleman Phylogenetic Analysis of Bacterial Communities in Mesophilic and Thermophilic Bioreactors Treating Pharmaceutical Wastewater Appl. Envir. Microbiol., September 1, 2000; 66(9): 3951 - 3959. [Abstract] [Full Text]
|
|
|
|
|
|
H. Imachi, Y. Sekiguchi, Y. Kamagata, A. Ohashi, and H. Harada Cultivation and In Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotrophic Methanogens in a Thermophilic Methanogenic Granular Sludge Appl. Envir. Microbiol., August 1, 2000; 66(8): 3608 - 3615. [Abstract] [Full Text]
|
|
|
|
|
|
S. Skirnisdottir, G. O. Hreggvidsson, S. Hjörleifsdottir, V. T. Marteinsson, S. K. Petursdottir, O. Holst, and J. K. Kristjansson Influence of Sulfide and Temperature on Species Composition and Community Structure of Hot Spring Microbial Mats Appl. Envir. Microbiol., July 1, 2000; 66(7): 2835 - 2841. [Abstract] [Full Text]
|
|
|
|
|
|
H. Urakawa, K. Kita-Tsukamoto, and K. Ohwada Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay, Japan, as determined by 16S rRNA gene analysis Microbiology, November 1, 1999; 145(11): 3305 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Chin, T. Lukow, and R. Conrad Effect of Temperature on Structure and Function of the Methanogenic Archaeal Community in an Anoxic Rice Field Soil Appl. Envir. Microbiol., June 1, 1999; 65(6): 2341 - 2349. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Sekiguchi, Y. Kamagata, K. Nakamura, A. Ohashi, and H. Harada Fluorescence In Situ Hybridization Using 16S rRNA-Targeted Oligonucleotides Reveals Localization of Methanogens and Selected Uncultured Bacteria in Mesophilic and Thermophilic Sludge Granules Appl. Envir. Microbiol., March 1, 1999; 65(3): 1280 - 1288. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
