| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Pathogenicity and Medical Microbiology |
Department of Gastrointestinal Infections, Statens Serum Institut, 5 Artillerivej, 2300 Copenhagen 5, Denmark1
Author for correspondence: Karen Angeliki Krogfelt. Tel: +45 3268 3745. Fax: +45 3268 3873. e-mail: kak{at}ssi.dk
Adhesion mediated by fimbriae is thought to play an important role in the pathogenesis of urinary tract infections (UTI) by Escherichia coli. The majority of clinical isolates of E. coli from UTI are able to express type 1 fimbriae. However, the importance of these fimbriae as a virulence factor has been controversial. To investigate the expression of type 1 fimbriae in vivo during UTI, mice were transurethrally infected with uropathogenic E. coli C175-94 and type 1 fimbrial expression was determined directly by two independent methods at 2 h, 1 d and 3 d after infection. By use of an assay combining in situ rRNA hybridization and immunofluorescence, all bacterial cells detected in urine, bladders and kidneys from mice sacrificed 1 and 3 d after onset of infection were found to express type 1 fimbriae. In contrast, the majority of cells in the suspension used for infection of mice and specimens from mice sacrificed 2 h after inoculation were found to be non-fimbriated. Similar results were obtained with a PCR assay revealing the orientation of the invertible promoter driving the transcription of type 1 fimbrial genes. Whilst the promoter in both ON and OFF positions could be amplified from the suspension used for infection and specimens from mice sacrificed 2 h after inoculation, at 1 and 3 d after onset of infection only the promoter in the ON orientation could be amplified. These results show that introduction of E. coli C175-94 into the mouse urinary tract leads to markedly enhanced expression of type 1 fimbriae.
Keywords: type 1 fimbriae, phase variation, urinary tract infection, in situ hybridization, immunofluorescence
Abbreviations: UTI, urinary tract infections
This article has been cited by other articles:
|
|
 |
|
  D. A. Rosen, J. S. Pinkner, J. M. Jones, J. N. Walker, S. Clegg, and S. J. Hultgren Utilization of an Intracellular Bacterial Community Pathway in Klebsiella pneumoniae Urinary Tract Infection and the Effects of FimK on Type 1 Pilus Expression Infect. Immun., July 1, 2008; 76(7): 3337 - 3345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Rosen, J. S. Pinkner, J. N. Walker, J. S. Elam, J. M. Jones, and S. J. Hultgren Molecular Variations in Klebsiella pneumoniae and Escherichia coli FimH Affect Function and Pathogenesis in the Urinary Tract Infect. Immun., July 1, 2008; 76(7): 3346 - 3356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Nowrouzian, V. Friman, I. Adlerberth, and A. E. Wold Reduced Phase Switch Capacity and Functional Adhesin Expression of Type 1-Fimbriated Escherichia coli from Immunoglobulin A-Deficient Individuals Infect. Immun., February 1, 2007; 75(2): 932 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bryan, P. Roesch, L. Davis, R. Moritz, S. Pellett, and R. A. Welch Regulation of Type 1 Fimbriae by Unlinked FimB- and FimE-Like Recombinases in Uropathogenic Escherichia coli Strain CFT073 Infect. Immun., February 1, 2006; 74(2): 1072 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. van der Woude and A. J. Baumler Phase and Antigenic Variation in Bacteria Clin. Microbiol. Rev., July 1, 2004; 17(3): 581 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Buckles, F. K. Bahrani-Mougeot, A. Molina, C. V. Lockatell, D. E. Johnson, C. B. Drachenberg, V. Burland, F. R. Blattner, and M. S. Donnenberg Identification and Characterization of a Novel Uropathogenic Escherichia coli-Associated Fimbrial Gene Cluster Infect. Immun., July 1, 2004; 72(7): 3890 - 3901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Kerrn, N. Frimodt-Moller, and F. Espersen Effects of Sulfamethizole and Amdinocillin against Escherichia coli Strains (with Various Susceptibilities) in an Ascending Urinary Tract Infection Mouse Model Antimicrob. Agents Chemother., March 1, 2003; 47(3): 1002 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Struve, C. Forestier, and K. A. Krogfelt Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection Microbiology, January 1, 2003; 149(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Gunther IV, J. A. Snyder, V. Lockatell, I. Blomfield, D. E. Johnson, and H. L. T. Mobley Assessment of Virulence of Uropathogenic Escherichia coli Type 1 Fimbrial Mutants in Which the Invertible Element Is Phase-Locked On or Off Infect. Immun., July 1, 2002; 70(7): 3344 - 3354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Schwan, J. L. Lee, F. A. Lenard, B. T. Matthews, and M. T. Beck Osmolarity and pH Growth Conditions Regulate fim Gene Transcription and Type 1 Pilus Expression in Uropathogenic Escherichia coli Infect. Immun., March 1, 2002; 70(3): 1391 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Culham, A. Lu, M. Jishage, K. A. Krogfelt, A. Ishihama, and J. M. Wood The osmotic stress response and virulence in pyelonephritis isolates of Escherichia coli: contributions of RpoS, ProP, ProU and other systems Microbiology, June 1, 2001; 147(6): 1657 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Graham, J. B. S. Leathart, S. J. Keegan, J. Pearson, A. Bint, and D. L. Gally Analysis of Escherichia coli Strains Causing Bacteriuria during Pregnancy: Selection for Strains That Do Not Express Type 1 Fimbriae Infect. Immun., February 1, 2001; 69(2): 794 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. NAUGHTON, G. GRANT, S. BARDOCZ, E. ALLEN-VERCOE, M. J. WOODWARD, and A. PUSZTAI Expression of type 1 fimbriae (SEF 21) of Salmonella enterica serotype Enteritidis in the early colonisation of the rat intestine J. Med. Microbiol., February 1, 2001; 50(2): 191 - 197. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
