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Environmental Microbiology |
Microbiology Laboratory1 and Synthetic Organic Chemistry Laboratory2, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Author for correspondence: Toshiaki Kudo. Tel: +81 48 467 9544. Fax: +81 48 462 4672. e-mail: tkudo{at}postman.riken.go.jp
Comamonas testosteroni TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the meta pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (mhpA), extradiol dioxygenase (mhpB), 2-keto-4-pentenoate hydratase (mhpD), acetaldehyde dehydrogenase (acylating) (mhpF), 4-hydroxy-2-ketovalerate aldolase (mhpE) and the meta cleavage compound hydrolase (mhpC), were found in this cluster, encoded in this order. mhpD and mhpF were separated by two genes, orf4 and orf5, which were not necessary for growth on 3HPP. The gene mhpR, encoding a putative transcriptional activator of the IclR family, was located adjacent to mhpA in the opposite orientation. Disruption of the mhpB or mhpR genes affected growth on 3HPP or trans-3-hydroxycinnamate. The mhpB and mhpC gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the meta cleavage compound produced from DHPP, respectively.
Keywords: 3-(3-hydroxyphenyl)propionate, trans-3-hydroxycinnamate, meta pathway, biodegradation, Comamonas testosteroni
Abbreviations: 3HCI, trans-3-hydroxycinnamate; 3HPA, 3-hydroxyphenylacetate; 3HPP, 3-(3-hydroxyphenyl)propionate; 3MC, 3-methylcatechol; 4MC, 4-methylcatechol; DHBP, 2,3-dihydroxybiphenyl; DHCI, trans-2,3-dihydroxycinnamate; DHPP, 3-(2,3-dihydroxyphenyl)propionate; CFE, cell-free extract
The DDBJ/EMBL/GenBank accession number for the sequence reported in this paper is AB024335.
a Present address: Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
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