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Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans

Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, UK¹

Author for correspondence: Geoffrey M. Gadd. Tel:+44 1382 344266. Fax:+44 1382 344275. e-mail: g.m.gadd{at}dundee.ac.uk

Polarography was used to measure the copper-binding ability of culture filtrates from a range of sulphate-reducing bacteria (SRB), including pure cultures and environmental isolates. Of those tested, Desulfococcus multivorans was shown to have the greatest copper-binding capacity and this organism was used for further experiments. Extracellular copper- and zinc-binding activities of Dc. multivorans culture filtrates from batch cultures increased over time and reached a maximum after 10 d growth. The culture filtrate was shown to bind copper reversibly and zinc irreversibly. Twelve-day-old Dc. multivorans culture filtrates were shown to have a copper-binding capacity of 3·64±0·33 µmol ml^-1 with a stability constant, log₁₀K, of 5·68±0·64 (n=4). The metal-binding compound was partially purified from culture growth media by dichloromethane extraction followed by HPLC using an acetonitrile gradient.

Keywords: sulphate-reducing bacteria, copper binding, zinc binding, metal binding, Desulfococcus multivorans

Abbreviations: SRB, sulphate-reducing bacteria

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