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Genetics and Molecular Biology |
Biotechnology Centre of Oslo and School of Pharmacy, University of Oslo, PO Box 1123, N-0349 Oslo, Norway1
Unité de Biochimie Microbienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France2
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium3
Institut für Mikrobiologie, J. W. Göethe Universität, Frankfurt, Germany4
Unité de Lutte Biologique, Institut National de la Recherche Agronomique, France5
Author for correspondence: Anne-Brit Kolstø. Tel: +47 22 95 84 60. Fax: +47 22 69 41 30. e-mail: annebko{at}biotek.uio.no
PlcR is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen Bacillus cereus and the entomopathogenic Bacillus thuringiensis, and is induced in cells entering stationary phase. Among the genes regulated by PlcR are: plcA, encoding phosphatidylinositol-specific phospholipase C (PI-PLC); plc, encoding phosphatidylcholine-preferring phospholipase C (PC-PLC); nhe, encoding the non-haemolytic enterotoxin; hbl, encoding haemolytic enterotoxin BL (HBL); and genes specifying a putativeS-layer like surface protein and a putative extracellular RNase. By analysing 37·1 kb of DNA sequence surrounding hbl, plcA and plcR, 28 ORFs were predicted. Three novel genes putatively regulated by PlcR and encoding a neutral protease (NprB), a subtilase family serine protease (Sfp) and a putative cell-wall hydrolase (Cwh) were identified. The corresponding sfp and cwh genes were located in the immediate upstream region of plcA and could both be regulated by a putative PlcR-binding site positioned between the inversely transcribed genes. Similarly, nprB was positioned directly upstream and transcribed in the opposite orientation to plcR. Genes surrounding plcA, plcR and hblCDAB that were lacking an upstream PlcR regulatory sequence did not appear to serve functions apparently related to PlcR and did not exhibit a conserved organization in Bacillus subtilis.
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Abbreviations: PC-PLC, phosphatidylcholine-preferring phospholipase C; PI-PLC, phosphatidylinositol-specific phospholipase C
The EMBL accession numbers for the sequences reported in this paper are given in Table 1.
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