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Genetics and Molecular Biology |
Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Apdo 85, 33300-Villaviciosa, Asturias, Spain1
Departamento de Biología Funcional, Area de Microbiología, Universidad de Oviedo, 33006-Oviedo, Spain2
Author for correspondence: Ana Rodríguez. Tel: +34 98 589 21 31. Fax: +34 98 589 22 33. e-mail: iplacsic{at}greencom.net
Synthesis of lactococcin 972 is plasmid-encoded. An operon composed of two genes that encode pre-bacteriocin and a putative immunity protein has been identified. The first gene encodes a 91-residue polypeptide that is exported via a sec-dependent system to give the mature 66-aa bacteriocin. The immunity protein is a 563-residue polypeptide with seven potential transmembrane domains. Two transcripts were observed from this region: one comprises the whole operon and is synthesized during the exponential phase of growth while the other, which corresponds just to the bacteriocin structural gene, presents a maximum in exponential cultures but is still present in late-stationary-phase cells.
Keywords: lactococcin 972, Lactococcus lactis, bacteriocin, plasmid-encoded genes
Abbreviations: LAB, lactic acid bacteria
The GenBank accession number for the sequence reported in this paper is AJ002203.
a Present address: TNO Nutrition and Food Research Institute, Department of Molecular Genetics and Gene Technology, PO Box 360, Utrechtseweg 48, 3700 AJ Zeist, The Netherlands.
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