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IS1626, a new IS900-related Mycobacterium avium insertion sequence

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada T6G 2H7¹

Author for correspondence: Dennis Y. Kunimoto. Tel: +1 780 407 1418. Fax: +1 780 4927521. e-mail: Dennis.Kunimoto{at}Ualberta.Ca

An insertion sequence designated IS1626 was isolated and characterized from a Mycobacterium avium clinical strain. IS1626 was detected by high-stringency hybridization with the pMB22/S12 probe from IS900 of Mycobacterium paratuberculosis. IS1626 is 1418 bp in size and has a G+C content of 65 mol%. It has neither terminal inverted repeats nor flanking direct repeats. Analysis of three IS1626 insertion sites in the M. avium strain and the corresponding potential insertion sites in two IS1626-free M. avium strains indicated a consensus sequence of CATGCN_(4–5)TCCTN₍₂₎G for IS1626 insertion. In the three clones examined, IS1626 has the same orientation with respect to this target site. IS1626 has two major ORFs. ORF1179 encodes a predicted protein of 393 amino acids. ORF930, on the complementary strand of ORF1179, encodes a protein of 310 amino acids. The Shine–Dalgarno sequence for ORF930 is partially located in the flanking region, similar to other IS900-related elements. Analysis of the comparable features of insertion sequences and their variable occurrence in related organisms is useful for studying the evolution of these elements and their hosts.

Keywords: mobile genetic elements, transposition, IS900, direct repeats, inverted repeats

Abbreviations: IS, insertion sequence; MAC, Mycobacterium avium complex

The GenBank accession number for the IS1626 sequence determined in this work is AF071067.

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