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The Q15H mutation enables Crh, a Bacillus subtilis HPr-like protein, to carry out some regulatory HPr functions, but does not make it an effective phosphocarrier for sugar transport

Unité de Biochimie Microbienne, CNRS URA 1300, Institut Pasteur,F-75724 Paris, France¹
Institut de Biologie et Chimie des Protéines, CNRS UPR 412, F-69367 Lyon Cedex 07, France²
Laboratoire de Génétique des Microorganismes, INRA-CNRS URA 1925,F-78850 Thiverval-Grignon, France³
Laboratoire de Chimie Bactérienne, CNRS UPR 9043, F-13009 Marseille, France⁴
Laboratoire de Biophysique, URA 491, Université Louis Pasteur,F-67401 Illkirch Cedex, France⁵

Author for correspondence: Josef Deutscher. Tel: +33 1 30 81 54 47. Fax: +33 1 30 81 54 57. e-mail: jdeu{at}platon.grignon.inra.fr

Crh of Bacillus subtilis exhibits 45% sequence identity when compared to histidine-containing protein (HPr), a phosphocarrier protein of the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS). Crh can be phosphorylated by ATP at the regulatory Ser-46 and similar to P-Ser-HPr, P-Ser-Crh plays a role in carbon-catabolite repression. The sequence around the phosphorylatable Ser-46 in Crh exhibits strong similarity to the corresponding sequence of HPr of Gram-positive and a few Gram-negative bacteria. In contrast, the catalytic His-15, the site of PEP-dependent phosphorylation in HPr, is replaced with a glutamine in Crh. When Gln-15 was exchanged for a histidyl residue, in vitro PEP-dependent enzyme I-catalysed phosphorylation of the mutant Crh was observed. However, expression of the crhQ15H mutant allele did not restore growth of a ptsH deletion strain on the PTS sugars glucose, fructose or mannitol or on the non-PTS sugar glycerol. In contrast, Q15H mutant Crh could phosphorylate the transcriptional activator LevR as well as LevD, the enzyme IIA of the fructose-specific lev-PTS, which together with enzyme I, HPr and LevE forms the phosphorylation cascade regulating induction of the lev operon via LevR. As a consequence, the constitutive expression from the lev promoter observed in a ptsH strain became inducible with fructose when the crhQ15H allele was expressed in this strain.

Keywords: PEP:sugar phosphotransferase system, HPr, Crh, catabolite repression

Abbreviations: FPr, fructose-specific HPr; HPr, histidine-containing protein; NPr, nitrogen-related HPr; PEP, phosphoenolpyruvate; PTS, PEP:sugar phosphotransferase system

^a Present address: Unité de Régulation de l’Expression Génétique, CNRS URA 1129, Institut Pasteur, F-75724 Paris, France.

^b Present address: Dept of Biochemistry, The Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1016, USA.

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