HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vannier-Santos, M. A. Articles by de Souza, W. Search for Related Content PubMed PubMed Citation Articles by Vannier-Santos, M. A. Articles by de Souza, W. Agricola Articles by Vannier-Santos, M. A. Articles by de Souza, W.

Impairment of sterol biosynthesis leads to phosphorus and calcium accumulation in Leishmania acidocalcisomes

Laboratório de Biologia Celular Parasitária, Programa de Biologia Celular e Parasitologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Bloco G, Ilha do Fundão, Rio de Janeiro, 21949-900, Brazil¹
Setor de Microscopia Eletrônica e Departamento de Microbiologia Geral, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil²
Laboratório de Química Biológica, Instituto Venezoelano de Investigaciones Científicas-IVIC, Venezuela³
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil⁴

Author for correspondence: Marcos A. Vannier-Santos. Tel: +55 21 260 6963. Fax: +55 21 280 8193. e-mail: vannier{at}biof.ufrj.br

The induction of the formation of inclusion vesicles in Leishmania amazonensis by the sterol biosynthesis inhibitors (SBI) ketoconazole and terbinafine has been reported previously. These compartments were recently identified as acidocalcisomes. By the use of electron spectroscopic imaging and energy loss spectroscopy, the presence of calcium, phosphorus and oxygen in the electron-dense inclusions located within the acidocalcisomes has been demonstrated. Endoplasmic reticulum cisternae formed membrane whorls which enclosed large portions of the cytoplasm and sometimes circumscribed acidocalcisomes. In addition, acid phosphatase activity, as well as the endocytic tracers horseradish peroxidase and gold-labelled transferrin and cystatin C were detected within these organelles in both SBI-treated and untreated parasites. These data suggest that impairment of sterol biosynthesis induces the biogenesis of acidocalcisomes and triggers an autophagic process that leads to intersection of the endosomal/lysosomal system with the acidocalcisomes.

Keywords: sterol biosynthesis inhibitors, ketoconazole, terbinafine, acidocalcisome, Leishmania

Abbreviations: ER, endoplasmic reticulum; ESI, electron spectroscopic imaging; SBI, sterol biosynthesis inhibitors

This article has been cited by other articles:

				M. A. Vannier-Santos, D. Menezes, M. F. Oliveira, and F. G. de Mello The putrescine analogue 1,4-diamino-2-butanone affects polyamine synthesis, transport, ultrastructure and intracellular survival in Leishmania amazonensis Microbiology, October 1, 2008; 154(10): 3104 - 3111. [Abstract] [Full Text] [PDF]

				S. Besteiro, D. Tonn, L. Tetley, G. H. Coombs, and J. C. Mottram The AP3 adaptor is involved in the transport of membrane proteins to acidocalcisomes of Leishmania J. Cell Sci., March 1, 2008; 121(5): 561 - 570. [Abstract] [Full Text] [PDF]

				M. J. Rodgers, J. P. Albanesi, and M. A. Phillips Phosphatidylinositol 4-Kinase III-{beta} Is Required for Golgi Maintenance and Cytokinesis in Trypanosoma brucei Eukaryot. Cell, July 1, 2007; 6(7): 1108 - 1118. [Abstract] [Full Text] [PDF]

				M. J. McConville, K. A. Mullin, S. C. Ilgoutz, and R. D. Teasdale Secretory Pathway of Trypanosomatid Parasites Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 122 - 154. [Abstract] [Full Text] [PDF]

				J. C. F. Rodrigues, M. Attias, C. Rodriguez, J. A. Urbina, and W. d. Souza Ultrastructural and Biochemical Alterations Induced by 22,26-Azasterol, a {Delta}24(25)-Sterol Methyltransferase Inhibitor, on Promastigote and Amastigote Forms of Leishmania amazonensis Antimicrob. Agents Chemother., February 1, 2002; 46(2): 487 - 499. [Abstract] [Full Text] [PDF]

				S. Dutoya, S. Gibert, G. Lemercier, X. Santarelli, D. Baltz, T. Baltz, and N. Bakalara A Novel C-terminal Kinesin Is Essential for Maintaining Functional Acidocalcisomes in Trypanosoma brucei J. Biol. Chem., December 21, 2001; 276(52): 49117 - 49124. [Abstract] [Full Text] [PDF]