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microbiology, Vol 145, 603-611, Copyright © 1999 by Society for General Microbiology
ARTICLES |
S Ruberg, A Puhler and A Becker
Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Germany.
The soil bacterium Sinorhizobium meliloti (Rhizobium meliloti) has the ability to produce the alternative exopolysaccharide galactoglucan (EPS II) in addition to succinoglycan (EPS I). In the wild-type strain EPS II production is induced by phosphate-limiting conditions or by extra copies of the exp gene cluster. Based on similarities to transcriptional regulators of the MarR family, an additional putative regulatory gene, expG, was identified in the exp gene cluster. Using exp-lacZ transcriptional fusions, a stimulating effect of extra copies of this expG gene on the transcription of all exp complementation groups was determined. Phosphate limitation also resulted in increased expression of the exp-lacZ fusions. This increase was reduced in strains characterized by a deletion of expG. The previously reported high level of exp gene transcription in a mucR mutant was further elevated under phosphate-limiting conditions. The expA, expD, expG and expE promoters contain sequences with similarities to the PHO box known as the PhoB-binding site in phosphate-regulated promoters in Escherichia coli. The S. meliloti phoB gene was required for the activation of exp gene expression under phosphate limitation, but not for induction of exp expression by MucR or ExpG.
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C. Bahlawane, B. Baumgarth, J. Serrania, S. Ruberg, and A. Becker Fine-Tuning of Galactoglucan Biosynthesis in Sinorhizobium meliloti by Differential WggR (ExpG)-, PhoB-, and MucR-Dependent Regulation of Two Promoters J. Bacteriol., May 15, 2008; 190(10): 3456 - 3466. [Abstract] [Full Text] [PDF]
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S. A. Glenn, N. Gurich, M. A. Feeney, and J. E. Gonzalez The ExpR/Sin Quorum-Sensing System Controls Succinoglycan Production in Sinorhizobium meliloti J. Bacteriol., October 1, 2007; 189(19): 7077 - 7088. [Abstract] [Full Text] [PDF]
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 M. Miller-Williams, P. C. Loewen, and I. J. Oresnik Isolation of salt-sensitive mutants of Sinorhizobium meliloti strain Rm1021 Microbiology, July 1, 2006; 152(7): 2049 - 2059. [Abstract] [Full Text] [PDF]
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B. Baumgarth, F. W. Bartels, D. Anselmetti, A. Becker, and R. Ros Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA Microbiology, January 1, 2005; 151(1): 259 - 268. [Abstract] [Full Text] [PDF]
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H. H. Hoang, A. Becker, and J. E. Gonzalez The LuxR Homolog ExpR, in Combination with the Sin Quorum Sensing System, Plays a Central Role in Sinorhizobium meliloti Gene Expression J. Bacteriol., August 15, 2004; 186(16): 5460 - 5472. [Abstract] [Full Text] [PDF]
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T. Danhorn, M. Hentzer, M. Givskov, M. R. Parsek, and C. Fuqua Phosphorus Limitation Enhances Biofilm Formation of the Plant Pathogen Agrobacterium tumefaciens through the PhoR-PhoB Regulatory System J. Bacteriol., July 15, 2004; 186(14): 4492 - 4501. [Abstract] [Full Text] [PDF]
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M. M. Marketon, S. A. Glenn, A. Eberhard, and J. E. Gonzalez Quorum Sensing Controls Exopolysaccharide Production in Sinorhizobium meliloti J. Bacteriol., January 1, 2003; 185(1): 325 - 331. [Abstract] [Full Text] [PDF]
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L. M. Moreira, J. D. Becker, A. Puhler, and A. Becker The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan Microbiology, September 1, 2000; 146(9): 2237 - 2248. [Abstract] [Full Text] [PDF]
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M. L. Summers, L. M. Botero, S. C. Busse, and T. R. McDermott The Sinorhizobium meliloti Lon Protease Is Involved in Regulating Exopolysaccharide Synthesis and Is Required for Nodulation of Alfalfa J. Bacteriol., May 1, 2000; 182(9): 2551 - 2558. [Abstract] [Full Text]
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K. E. Mendrygal and J. E. González Environmental Regulation of Exopolysaccharide Production in Sinorhizobium meliloti J. Bacteriol., February 1, 2000; 182(3): 599 - 606. [Abstract] [Full Text]
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