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Seattle Biomedical Research Institute, 4 Nickerson St, Seattle, WA 98109, USA
ABSTRACT
Isolates of the Mycobacterium avium-intracellulare complex (MAC) have long been known to segregate into transparent, opaque and rough colony morphotypes that differ from each other in clinically important parameters including drug susceptibility and virulence. Here the authors report additional morphotypic variation that occurs on two levels: interspecific (between M. avium and M. intracellulare) and intraspecific (within individual M. avium isolates). Clinical isolates of M. avium grown on Congo red (CR) plates formed red, pink or mixed (red and white) opaque colonies, while M. intracellulare isolates formed purely white opaque colonies. A quantitative CR binding assay showed that this interspecific differential applies to transparent as well as opaque colony variants; however, it was less pronounced among laboratory reference strains than among recent clinical isolates. Opaque colonies of M. avium isolates with ‘mixed’ phenotypes segregated into stable opaque red and white variants with shared IS1245 banding patterns (intraspecific segregation). White segregants of M. avium were more flocculent and significantly more resistant to ciprofloxacin and rifamycin drugs than were red segregants. Thus, cultivation on CR agar revealed a previously unknown multi-drug resistant colony morphotype of M. avium.
Author for correspondence: Gerard A. Cangelosi. Tel: +1 206 284 8846. Fax: +1 206 284 0313. e-mail: cangelos@u.washington.edu
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