|
|
||||||||
microbiology, Vol 145, 1325-1333, Copyright © 1999 by Society for General Microbiology
ARTICLES |
I Williams, F Paul, D Lloyd, R Jepras, I Critchley, M Newman, J Warrack, T Giokarini, AJ Hayes, PF Randerson, WA Venables
The techniques of flow cytometry, scanning and transmission electron microscopy, and confocal scanning laser microscopy were used to study the physiology of Staphylococcus aureus in the early stages of surface-attached culture, and to make direct comparisons with planktonic bacteria grown under the same conditions. Attached bacteria growing in nutrient-rich batch culture were found to go through the same growth phases as equivalent planktonic cultures, but with an exponential growth rate of about half that of the planktonic bacteria. Viability of attached bacteria was very high (around 100%) throughout the first 24 h of growth. The size and protein content of attached bacteria varied with growth phase, and both measurements were always smaller than in planktonic bacteria at equivalent growth phases. Respiratory activity per bacterium, as measured by flow cytofluorimetry, and corrected for cell volume, peaked very early in attached cultures (before the first cell division) and declined from then on, whereas in planktonic bacteria it peaked in late exponential phase. Attached and planktonic bacteria showed thicker cell walls in stationary phase than in exponential phase. Membrane potentials of planktonic and attached bacteria were similar in stationary phase, but were much lower in exponential-phase attached cells than in the equivalent planktonic cells. It is apparent that a range of significant physiological adaptations occur during the early phases of attached growth.
This article has been cited by other articles:
![]() |
M. Ryder Evidence-Based Practice in the Management of Vascular Access Devices for Home Parenteral Nutrition Therapy JPEN J Parenter Enteral Nutr, January 1, 2006; 30(1_suppl): S82 - S93. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Taniguchi, T. Ono, K. Murakami, D. Viducic, S. Kayama, K. Hirota, K. Nemoto, and Y. Miyake Novel Pseudomonas aeruginosa Gene That Suppresses Tolerance to Carbapenems Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2997 - 3001. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. B. I. Luppens, M. W. Reij, R. W. L. van der Heijden, F. M. Rombouts, and T. Abee Development of a Standard Test To Assess the Resistance of Staphylococcus aureus Biofilm Cells to Disinfectants Appl. Envir. Microbiol., September 1, 2002; 68(9): 4194 - 4200. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Wolz, C. Goerke, R. Landmann, W. Zimmerli, and U. Fluckiger Transcription of Clumping Factor A in Attached and Unattached Staphylococcus aureus In Vitro and during Device-Related Infection Infect. Immun., June 1, 2002; 70(6): 2758 - 2762. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Campanac, L. Pineau, A. Payard, G. Baziard-Mouysset, and C. Roques Interactions between Biocide Cationic Agents and Bacterial Biofilms Antimicrob. Agents Chemother., May 1, 2002; 46(5): 1469 - 1474. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. D. Ehrlich, R. Veeh, X. Wang, J. W. Costerton, J. D. Hayes, F. Z. Hu, B. J. Daigle, M. D. Ehrlich, and J. C. Post Mucosal Biofilm Formation on Middle-Ear Mucosa in the Chinchilla Model of Otitis Media JAMA, April 3, 2002; 287(13): 1710 - 1715. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. B. Budde and M. Jakobsen Real-Time Measurements of the Interaction between Single Cells of Listeria monocytogenes and Nisin on a Solid Surface Appl. Envir. Microbiol., August 1, 2000; 66(8): 3586 - 3591. [Abstract] [Full Text] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |