| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
microbiology, Vol 145, 1421-1429, Copyright © 1999 by Society for General Microbiology
ARTICLES |
PS Solomon, AL Shaw, I Lane, GR Hanson, T Palmer and AG McEwan
Department of Microbiology, The University of Queensland, Brisbane 4072, Australia
The DMSO reductase of Rhodobacter capsulatus contains a pterin molybdenum cofactor (Moco) and is located in the periplasm. DNA sequence analysis identified four genes involved in the biosynthesis of the Moco (moaA, moaD, moeB and moaC) immediately downstream of the dor (DMSO respiratory) gene cluster. Rhodobacter capsulatus MoaA was expressed in Escherichia coli as a His6-tagged protein. Although, the expressed protein formed inclusion bodies, EPR spectroscopy showed that MoaA contains a [3Fe--4S] cluster. A moaA mutant was constructed and its phenotype indicates that the Moco biosynthetic gene cluster downstream of the dor operon is specific for the biogenesis of DMSO reductase. Two forms of DMSO reductase were purified by immunoaffinity chromatography from the moaA mutant. A mature form of DMSO reductase was located in the periplasm and a precursor form was found in the cytoplasm.
This article has been cited by other articles:
|
|
 |
|
  M. Neumann, W. Stocklein, and S. Leimkuhler Transfer of the Molybdenum Cofactor Synthesized by Rhodobacter capsulatus MoeA to XdhC and MobA J. Biol. Chem., September 28, 2007; 282(39): 28493 - 28500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hanzelmann, H. L. Hernandez, C. Menzel, R. Garcia-Serres, B. H. Huynh, M. K. Johnson, R. R. Mendel, and H. Schindelin Characterization of MOCS1A, an Oxygen-sensitive Iron-Sulfur Protein Involved in Human Molybdenum Cofactor Biosynthesis J. Biol. Chem., August 13, 2004; 279(33): 34721 - 34732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hanzelmann, G. Schwarz, and R. R. Mendel Functionality of Alternative Splice Forms of the First Enzymes Involved in Human Molybdenum Cofactor Biosynthesis J. Biol. Chem., May 17, 2002; 277(21): 18303 - 18312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Kappler, W. M. Huston, and A. G. McEwan Control of dimethylsulfoxide reductase expression in Rhodobacter capsulatus: the role of carbon metabolites and the response regulators DorR and RegA Microbiology, February 1, 2002; 148(2): 605 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sabaty, C. Avazeri, D. Pignol, and A. Vermeglio Characterization of the Reduction of Selenate and Tellurite by Nitrate Reductases Appl. Envir. Microbiol., November 1, 2001; 67(11): 5122 - 5126. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Zheng, R. Hehn, and P. Zuber Mutational Analysis of the sbo-alb Locus of Bacillus subtilis: Identification of Genes Required for Subtilosin Production and Immunity J. Bacteriol., June 1, 2000; 182(11): 3266 - 3273. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
