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Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum

K. J. Edwards

Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

ABSTRACT

The acetan biosynthetic pathway in Acetobacter xylinum is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an Acetobacter strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, aceP, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with β-D-glucosyl transferases from a number of different organisms. Disruption of aceP in strain CKE5 confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.

Author for correspondence: A. M. Griffin. Tel: + 44 1603 255354. Fax: + 44 1603 507723. e-mail: annette.griffin@bbsrc.ac.uk

Present address: Central Public Health Laboratory, 61 Colindale Avenue, Colindale, London, NW9 5HT, UK.

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