Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 145 (1999), 1499-1506
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Edwards, K. J.
Right arrow Articles by Griffin, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edwards, K. J.
Right arrow Articles by Griffin, A. M.
Agricola
Right arrow Articles by Edwards, K. J.
Right arrow Articles by Griffin, A. M.

microbiology, Vol 145, 1499-1506, Copyright © 1999 by Society for General Microbiology

ARTICLES

Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum

KJ Edwards, AJ Jay, IJ Colquhoun, VJ Morris, MJ Gasson and AM Griffin
Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

The acetan biosynthetic pathway in Acetobacter xylinum is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an Acetobacter strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, aceP, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with beta-D-glucosyl transferases from a number of different organisms. Disruption of aceP in strain CKE5 confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
L. Wang, W. Qu, and P. R. Reeves
Sequence Analysis of Four Shigella boydii O-Antigen Loci: Implication for Escherichia coli and Shigella Relationships
Infect. Immun., November 1, 2001; 69(11): 6923 - 6930.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Garinot-Schneider, A. C. Lellouch, and R. A. Geremia
Identification of Essential Amino Acid Residues in the Sinorhizobium meliloti Glucosyltransferase ExoM
J. Biol. Chem., September 29, 2000; 275(40): 31407 - 31413.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1999 Society for General Microbiology.