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70 analogue modulate promoter recognition
1 Department of Laboratory Medicine and the Francis I. Proctor Foundation, San Francisco, CA 94143, USA
2 Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA
3 Program in Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA
Author for correspondence: Richard S. Stephens. Tel: -1 510 643 9900. Fax: -1510 643 5676. e-mail: rss@uclink4.berkeley.edu
ABSTRACT
Genes from the eubacterium Chlamydia typically do not share promoter consensus sequences with those of Escherichia coli and are not expressed when cloned in E. coli; nevertheless, the major 
-subunit identified from Chlamydia trachomatis has nearly identical amino acid sequence to E. coli 70 in regions that contact DNA. Following expression of the chlamydial -subunit gene in E. coli, expression was specifically initiated from chlamydial promoter regions. Selective recognition of chlamydial promoters by holoenzyme was dependent upon the structure of the promoter DNA coupled with novel amino-and carboxyl-terminal extensions of the chlamydial -subunit.
Present address: Centre for Moelcular Biotechnology, School of Life Science, Queensland University of Technology, Brisbane 4001, Australia.
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