| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331-3804, USA
Present address: NADC, 2300 Dayton Ave, Ames, IA 50010, USA.
Author for correspondence: Daniel D. Rockey. Tel: +1 541 737 1848. Fax: + 1 541 737 0496. e-mail: rockeyd@ucs.orst.edu
ABSTRACT
A primate model system was used to identify Chlamydia trachomatis antigens uniquely recognized in the context of infection. Serum antibody titres were measured in cynomolgus monkeys challenged urethrally with C. trachomatis serovar L2 elementary bodies (EBs). High-titre sera from these primates were used, in parallel with antisera against killed C. trachomatis EBs, to differentially screen an expression library of C. trachomatis serovar L2 DNA. Four clones were recognized only by antisera from infected monkeys. Sequence analysis revealed that three of these immunoreactive clones overlap a common ORF, designated ORF D242 (encoding p242), in the C. trachomatis genome database. The fourth clone contains two complete ORFs, each encoding 32 kDa proteins that share identity with Treponema pallidum TroA and TroB (ORFs D067 and D068 in the C. trachomatis database, respectively). lmmunoblot analysis of Escherichia coli lysates expressing C. trachomatis TroA, TroB and p242 fusion proteins showed that p242 and TroA, but not TroB, were detected by the sera collected from infected primates. Antibodies directed at TroA and p242 were also detected in sera from several C. trachomatis-infected patients, demonstrating that these proteins are also recognized by humans following infection. Immunoblot analysis with antibody against TroA and p242 also demonstrated that both antigens are present in higher abundance in infected ChoK1 cells relative to purified C. trachomatis EBs. Immunofluorescence microscopy shows that TroA and p242 are both localized to intracellular developmental forms at the margins of growing inclusions. Collectively, these studies identify two C. trachomatis proteins that are under-represented in EBs and are recognized uniquely in the context of infection.
The GenBank accession numbers for the sequences reported in this paper are AF077009 and AF077010.
Abbreviations: EB, elementary body; 
-EB, anti-killed C. trachomatis EB sera; MBP, maltose-binding protein; MOMP, major outer-membrane protein; PI, post-infection; RB, reticulate body.
This article has been cited by other articles:
|
|
 |
|
  J. D. Miller, M. S. Sal, M. Schell, J. D. Whittimore, and J. E. Raulston Chlamydia trachomatis YtgA is an iron-binding periplasmic protein induced by iron restriction Microbiology, September 1, 2009; 155(9): 2884 - 2894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mukhopadhyay, A. Akmal, A. C. Stewart, R.-c. Hsia, and T. D. Read Identification of Bacillus anthracis Spore Component Antigens Conserved across Diverse Bacillus cereus sensu lato Strains Mol. Cell. Proteomics, June 1, 2009; 8(6): 1174 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dumetz, E. Duchaud, S. E. LaPatra, C. Le Marrec, S. Claverol, M.-C. Urdaci, and M. Le Henaff A Protective Immune Response Is Generated in Rainbow Trout by an OmpH-Like Surface Antigen (P18) of Flavobacterium psychrophilum. Appl. Envir. Microbiol., July 1, 2006; 72(7): 4845 - 4852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Garmory and R. W. Titball ATP-Binding Cassette Transporters Are Targets for the Development of Antibacterial Vaccines and Therapies Infect. Immun., December 1, 2004; 72(12): 6757 - 6763. [Full Text] [PDF]
|
|
|
|
|
|
P. M. Bavoil, R.-c. Hsia, and D. M. Ojcius Closing in on Chlamydia and its intracellular bag of tricks Microbiology, November 1, 2000; 146(11): 2723 - 2731. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
