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Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus

Department of Human Genetics, University Medical School of Debrecen, H-4012 Debrecen Nagyerdei körút 98, Hungary¹
Department of Medicinal Chemistry, Albert Szent-Györgyi Medical University, H-6720 Szeged Dóm tér 8, Hungary²
Department of Biochemistry, Leiden University, Gorlaeus Laboratory, PO Box 9502, 2300 RA Leiden, The Netherlands³

Author for correspondence: Sándor Biró. Tel: +36 52 416 531. Fax: +36 52 416 531. e-mail: sbiro{at}jaguar.dote.hu

The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34523 Da. The mature extracellular 286 amino acid protein (31038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

Keywords: extracellular signalling, cell differentiation, sporulation, autoregulator, Streptomyces

Abbreviations: DIG, digoxigenin; ESI, electrospray ionization

This paper is dedicated to the memory of Professor Gábor Szabó.

The GenBank accession number for the sequence reported in this paper is AF103943.

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