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Genetics and Molecular Biology |
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1
Author for correspondence: Raimund Eck. Tel: +49 3641 656852. Fax: +49 3641 656652. e-mail: reck{at}pmail.hki-jena.de
A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70·5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant (fet3
/fet3) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3 mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3 mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.
Keywords: multicopper oxidase, Candida albicans, gene disruption, pathogenicity, iron uptake
Abbreviations: BPA, bathophenanthrolinedisulfonic acid
b The EMBL accession number for the sequence reported in this paper is Y09329.
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