| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK1
Author for correspondence: J. M. Ketley. Tel: +44 116 252 3434. Fax: +44 116 252 3378. e-mail: ket{at}leicester.ac.uk
To survive and multiply in different environments, Vibrio cholerae has to co-ordinately regulate the expression of genes involved in adaptive responses. In many pathogens, adaptive responses, including pathogenic responses, are regulated by two-component regulator (TCR) systems. It is likely that members of a TCR family play a role in the regulation of processes involved in intestinal colonization, and therefore pathogenesis, in V. cholerae. We have identified and characterized a TCR system of V. cholerae: this system is a homologue of Escherichia coli PhoBR. The presence of a putative Pho box suggests that the V. cholerae phoBR operon is regulated by inorganic phosphate levels. The phoR and phoB genes are organized the same way as in E. coli. Mutation of the V. cholerae phoB gene affected the expression of the putative Pho regulon, including PhoA, but did not affect the production of cholera toxin. V. cholerae phoB mutants are less able to colonize rabbit intestine than wild-type V. cholerae. The addition of inorganic phosphate at a high concentration to the inoculum only partially restored the ability of the mutants to colonize the intestine, suggesting that the V. cholerae Pho regulon in vivo may not be regulated by inorganic phosphate levels alone.
Keywords: Vibrio cholerae, PhoBR, two-component, regulation, colonization
Abbreviations: CT, cholera toxin; HK, histidine kinase; RILAT, rabbit ileal loop anastomosis test; RR, response regulator; TCR, two-component regulator; TGLP, Tris/glucose low phosphate medium; TGHP, Tris/glucose high phosphate medium
The GenBank accession number for the sequence reported in this paper is AF043352.
a Present address: Laboratorio de Fisiologia Celular, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21949-900, Rio de Janeiro, Brazil.
b Present address: Deparment of Veterinary Pathology, Glasgow University, Bearsden, Glasgow G61 1QH, UK.
This article has been cited by other articles:
|
|
 |
|
  G. M. Ferreira and B. Spira The pst operon of enteropathogenic Escherichia coli enhances bacterial adherence to epithelial cells Microbiology, July 1, 2008; 154(7): 2025 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-J. Lombardo, J. Michalski, H. Martinez-Wilson, C. Morin, T. Hilton, C. G. Osorio, J. P. Nataro, C. O. Tacket, A. Camilli, and J. B. Kaper An in vivo expression technology screen for Vibrio cholerae genes expressed in human volunteers PNAS, November 13, 2007; 104(46): 18229 - 18234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gebhard, S. L. Tran, and G. M. Cook The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate. Microbiology, November 1, 2006; 152(Pt 11): 3453 - 3465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. K. Jahid, A. J. Silva, and J. A. Benitez Polyphosphate Stores Enhance the Ability of Vibrio cholerae To Overcome Environmental Stresses in a Low-Phosphate Environment Appl. Envir. Microbiol., November 1, 2006; 72(11): 7043 - 7049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Monds, P. D. Newell, J. A. Schwartzman, and G. A. O'Toole Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1. Appl. Envir. Microbiol., March 1, 2006; 72(3): 1910 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Lamarche, C. M. Dozois, F. Daigle, M. Caza, R. Curtiss III, J. D. Dubreuil, and J. Harel Inactivation of the Pst System Reduces the Virulence of an Avian Pathogenic Escherichia coli O78 Strain Infect. Immun., July 1, 2005; 73(7): 4138 - 4145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. John, I. T. Kudva, R. W. Griffin, A. W. Dodson, B. McManus, B. Krastins, D. Sarracino, A. Progulske-Fox, J. D. Hillman, M. Handfield, et al. Use of In Vivo-Induced Antigen Technology for Identification of Escherichia coli O157:H7 Proteins Expressed during Human Infection Infect. Immun., May 1, 2005; 73(5): 2665 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Collins, R. P. Kawakami, B. M. Buddle, B. J. Wards, and G. W. de Lisle Different susceptibility of two animal species infected with isogenic mutants of Mycobacterium bovis identifies phoT as having roles in tuberculosis virulence and phosphate transport Microbiology, November 1, 2003; 149(11): 3203 - 3212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Srinivasa Rao, T. M. Lim, and K. Y. Leung Functional Genomics Approach to the Identification of Virulence Genes Involved in Edwardsiella tarda Pathogenesis Infect. Immun., March 1, 2003; 71(3): 1343 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Dozois, F. Daigle, and R. Curtiss III Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain PNAS, January 7, 2003; 100(1): 247 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lebens, P. Lundquist, L. Soderlund, M. Todorovic, and N. I. A. Carlin The nptA Gene of Vibrio cholerae Encodes a Functional Sodium-Dependent Phosphate Cotransporter Homologous to the Type II Cotransporters of Eukaryotes J. Bacteriol., August 15, 2002; 184(16): 4466 - 4474. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
