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Pathogenicity and Medical Microbiology |
Département de Microbiologie Médicale et Moléculaire, Unité de Bactériologie, CHU Bretonneau, 37044 Tours Cedex 1, France1
Institute of Molecular Evolutionary Genetics, Pennsylvania State University Park, PA 16801, USA2
Centre Héliomarin, 19 Boulevard Félix Faure, 17370 Saint Trojan-les-Bains, France3
Author for correspondence: Roland Quentin. Tel: +33 2 47 47 80 56. Fax: +33 2 47 47 38 12. e-mail: quentin{at}pop.med.univ-tours.fr
Each of 314 strains of Pseudomonas aeruginosa recovered from 87 French cystic fibrosis (CF) patients was typed by multilocus enzyme electrophoresis to investigate the genetic diversity, the relatedness and the molecular epidemiology of strains isolated from cases of chronic pulmonary colonization. Comparison of allele profiles at 18 enzyme loci identified 17 electrophoretic types (ETs). Of the 314 isolates, 290 (92%) were either ET1 (n=127) or ET2 (n=163), which differed only at the shikimate dehydrogenase (SKD) locus. The mean genetic diversity (H) was 0·138. These results suggest that there is cross-colonization between patients and/or that two predominant groups of strains are able to colonize French CF patients. Sequential isolates collected from 18 patients during a period of 12–28 months were analysed to assess genomic variability and its relationship to clinical outcome. Six patients were colonized by a stable strain. For the others, double infections or changes in colonization over time were observed. No relationships were detected between the clinical outcome and the persistence of stable isolates, the emergence of transient superinfecting variants, the presence of multiple ETs or the shift of ET during the monitoring.
Keywords: multilocus enzyme electrophoresis, MLEE, electrophoretic types, cystic fibrosis, P. aeruginosa
Abbreviations: CF, cystic fibrosis; ET, electrophoretic type; FIGE, field-inversion gel electrophoresis; MLEE, multilocus enzyme electrophoresis; RAPD, random amplified polymorphic DNA
a Present address: Laboratoire de Bactériologie-Virologie-Hygiène, Unité de Bactériologie, Centre Hospitalier Universitaire Dupuytren, 87042 Limoges Cedex, France.
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