HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gonzalez, P. Articles by Labarère, J. Search for Related Content PubMed PubMed Citation Articles by Gonzalez, P. Articles by Labarère, J. Agricola Articles by Gonzalez, P. Articles by Labarère, J.

Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains

Laboratory of Molecular Genetics and Breeding of Cultivated Mushrooms, INRA–University Victor Segalen Bordeaux 2, BP 81, 33883 Villenave d’Ornon Cédex, France¹

Author for correspondence: Jacques Labarère. Tel: +33 5 56 84 31 69. Fax: +33 5 56 84 31 79. e-mail: labarere{at}bordeaux.inra.fr

A comparative study of the V4, V6 and V9 domains of the mitochondrial small-subunit (SSU) rRNA was conducted to evaluate the use of these sequences to investigate phylogenetic relatedness within the genus Pleurotus. The PCR products encompassing these regions from 48 isolates belonging to 16 Pleurotus species were sequenced and compared. From this comparison, the length and sequence of the three domains were found to be constant within a species. Significant inter-species variations due to insertion/deletion events were found, in most cases occurring in regions not directly involved in the maintainance of the standard SSU rRNA secondary structure. Phylogenetic analysis based upon these mitochondrial sequences was in agreement with relationships previously established by morphological descriptions and with previous studies based upon the nuclear genome or isozymes; moreover such analysis resolved some ambiguities in earlier analyses. It was confirmed that P. ostreatus and P. florida represent a single species, as well as P. pulmonarius and P. sajor-caju. The phylogenetic analysis also made it possible to assess the relative positions of P. rattenburyi, P. lampas, P. sapidus, P. colombinus and P. eryngii. The results clearly showed that sequences of the V4, V6 and V9 domains of the mitochondrial SSU rRNA could provide good markers for use in the taxonomy and phylogeny of species of Basidiomycota. Because of their nucleotide conservation, the major advantage of these species-specific markers was the possibility to study only one isolate from each species to determine phylogenetic relatedness.

Keywords: Basidiomycota, mitochondrial SSU rRNA, Pleurotus, taxonomy, , phylogeny

Abbreviations: ITS, internal transcribed spacer; SSU, small subunit

The GenBank accession numbers for the sequences reported in this paper are given in Methods.

This article has been cited by other articles:

				Z.-H. Yang, J.-X. Huang, and Y.-J. Yao Autoscreening of Restriction Endonucleases for PCR-Restriction Fragment Length Polymorphism Identification of Fungal Species, with Pleurotus spp. as an Example Appl. Envir. Microbiol., December 15, 2007; 73(24): 7947 - 7958. [Abstract] [Full Text] [PDF]

				G. I. Zervakis, G. Venturella, and K. Papadopoulou Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters Microbiology, November 1, 2001; 147(11): 3183 - 3194. [Abstract] [Full Text] [PDF]