| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Physiology and Growth |
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, 79104 Freiburg, Germany1
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, and Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Str., 35043 Marburg, Germany2
Institut für Mikrobiologie, Universität Osnabrück, Barbarastr. 11, 49060 Osnabrück, Germany3
Author for correspondence: Dieter Jahn. Tel: +49 761 203 6060. Fax: +49 761 203 6096. e-mail: jahndiet{at}ruf.uni-freiburg.de
After a shift of Bacillus subtilis from aerobic to anaerobic growth conditions, nitrate ammonification and various fermentative processes replace oxygen-dependent respiration. Cell-free extracts prepared from wild-type B. subtilis and from mutants of the regulatory loci fnr and resDE grown under aerobic and various anaerobic conditions were compared by two-dimensional gel electrophoresis. Proteins involved in the adaptation process were identified by their N-terminal sequence. Induction of cytoplasmic lactate dehydrogenase (LctE) synthesis under anaerobic fermentative conditions was dependent on fnr and resDE. Anaerobic nitrate repression of LctE formation required fnr-mediated expression of narGHJI, encoding respiratory nitrate reductase. Anaerobic induction of the flavohaemoglobin Hmp required resDE and nitrite. The general anaerobic induction of ywfI, encoding a protein of unknown function, was modulated by resDE and fnr. The ywfI gene shares its upstream region with the pta gene, encoding the fermentative enzyme acetyl-CoA:orthophosphate acetyltransferase. Anaerobic repression of the synthesis of a potential membrane-associated NADH dehydrogenase (YjlD, Ndh), and anaerobic induction of fructose-1,6-bisphosphate aldolase (FbaA) and dehydrolipoamide dehydrogenase (PhdD, Lpd) formation, did not require fnr or resDE participation. Synthesis of glycerol kinase (GlpK) was decreased under anaerobic conditions. Finally, the effect of anaerobic stress induced by the immediate shift from aerobic to strictly anaerobic conditions was analysed. The induction of various systems for the utilization of alternative carbon sources such as inositol (IolA, IolG, IolH, IolI), melibiose (MelA) and 6-phospho-
-glucosides (GlvA) indicated a catabolite-response-like stress reaction.
Keywords: anaerobic growth, Bacillus subtilis, two-dimensional gel electrophoresis, stress response
This paper is dedicated to Professor Dr R. K. Thauer, Marburg, on the occasion of his 60th birthday.
This article has been cited by other articles:
|
|
 |
|
  N.-P. Hua, A. Kanekiyo, K. Fujikura, H. Yasuda, and T. Naganuma Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1243 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fuchs, J. Pane-Farre, C. Kohler, M. Hecker, and S. Engelmann Anaerobic Gene Expression in Staphylococcus aureus J. Bacteriol., June 1, 2007; 189(11): 4275 - 4289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. B. Thomaides, E. J. Davison, L. Burston, H. Johnson, D. R. Brown, A. C. Hunt, J. Errington, and L. Czaplewski Essential Bacterial Functions Encoded by Gene Pairs J. Bacteriol., January 15, 2007; 189(2): 591 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gyan, Y. Shiohira, I. Sato, M. Takeuchi, and T. Sato Regulatory Loop between Redox Sensing of the NADH/NAD+ Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis. J. Bacteriol., October 1, 2006; 188(20): 7062 - 7071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hartig, A. Hartmann, M. Schatzle, A. M. Albertini, and D. Jahn The Bacillus subtilis nrdEF Genes, Encoding a Class Ib Ribonucleotide Reductase, Are Essential for Aerobic and Anaerobic Growth Appl. Envir. Microbiol., August 1, 2006; 72(8): 5260 - 5265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Larsson, A. Rogstam, and C. von Wachenfeldt Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis Microbiology, October 1, 2005; 151(10): 3323 - 3335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hartig, H. Geng, A. Hartmann, A. Hubacek, R. Munch, R. W. Ye, D. Jahn, and M. M. Nakano Bacillus subtilis ResD Induces Expression of the Potential Regulatory Genes yclJK upon Oxygen Limitation J. Bacteriol., October 1, 2004; 186(19): 6477 - 6484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rosenkrands, R. A. Slayden, J. Crawford, C. Aagaard, C. E., I. Barry, and P. Andersen Hypoxic Response of Mycobacterium tuberculosis Studied by Metabolic Labeling and Proteome Analysis of Cellular and Extracellular Proteins J. Bacteriol., July 1, 2002; 184(13): 3485 - 3491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Oosthuizen, B. Steyn, J. Theron, P. Cosette, D. Lindsay, A. von Holy, and V. S. Brozel Proteomic Analysis Reveals Differential Protein Expression by Bacillus cereus during Biofilm Formation Appl. Envir. Microbiol., June 1, 2002; 68(6): 2770 - 2780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamamoto, M. Serizawa, J. Thompson, and J. Sekiguchi Regulation of the glv Operon in Bacillus subtilis: YfiA (GlvR) Is a Positive Regulator of the Operon That Is Repressed through CcpA and cre J. Bacteriol., September 1, 2001; 183(17): 5110 - 5121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Ye, W. Tao, L. Bedzyk, T. Young, M. Chen, and L. Li Global Gene Expression Profiles of Bacillus subtilis Grown under Anaerobic Conditions J. Bacteriol., August 15, 2000; 182(16): 4458 - 4465. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
