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migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-mass lipopolysaccharide

Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA¹
Department of Microbiology, University of Guelph, Guelph, Ontario, Canada²
Department of Biological Sciences, University of Calgary, Calgary, Canada³

Author for correspondence: Shouguang Jin. Tel: +1 352 392 8323. Fax: +1 352 392 3133. e-mail: sjin{at}mgm.ufl.edu

Pseudomonas aeruginosa is an opportunistic human pathogen which poses a major threat to patients with cystic fibrosis (CF). Excessive amounts of mucus present in the lungs of CF patients promotes the colonization of P. aeruginosa. The migA gene, encoding a putative glycosyltransferase, has been shown to be highly inducible by respiratory mucus derived from CF patients. In this study, it is further demonstrated by population transcript analysis that the migA gene is highly expressed in the CF lung environment. Deletion analysis of the migA promoter identified a las-box-like sequence commonly found in promoters that are responsive to quorum sensing regulation. Further analysis of migA expression in quorum-sensing-defective strains, as well as its expression in response to autoinducer molecules, demonstrated that migA is regulated by the RhlI/RhlR quorum sensing regulatory system. Functionally, as the MigA sequence homology data suggested, the migA gene indeed affects the structure of LPS in P. aeruginosa. Increased expression of the migA gene results in a loss of core-plus-one LPS, while having no obvious effect on the long-chain O-antigen-bearing LPS. Although the exact biological role of the core-plus-one LPS is not clear, these experimental results suggest that migA up-regulation in the CF lung environment is part of the adaptive response which confers on P. aeruginosa a survival advantage.

Keywords: migA, mucus, LPS and quorum response

Abbreviations: CF, cystic fibrosis; C12-HSL, 3-oxo-dodecanoyl homoserine lactone; C4-HSL, butanoyl homoserine lactone

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