Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 146 (2000), 2531-2541
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaufman, M. R.
Right arrow Articles by Jin, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaufman, M. R.
Right arrow Articles by Jin, S.
Agricola
Right arrow Articles by Kaufman, M. R.
Right arrow Articles by Jin, S.
Microbiology (2000), 146, 2531-2541.
© 2000 Society for General Microbiology

Pseudomonas: Biology and Diversity

Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of ExoS

Melissa R. Kaufman1, Jinghua Jia1,2, Lin Zeng1,2, Unhwan Ha1,2, Marie Chow1 and Shouguang Jin1,2

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1
Department of Molecular Genetics and Microbiology, PO Box 100266, University of Florida, Gainesville, FL 32610, USA2

Author for correspondence: Shouguang Jin. Tel: +1 352 392 8323. Fax: +1 352 392 3133. e-mail: sjin{at}mgm.ufl.edu

Pseudomonas aeruginosa is an opportunistic bacterial pathogen that primarily infects immunocompromised individuals and patients with cystic fibrosis. Using a tissue culture system, invasive strains of P. aeruginosa were discovered to induce apoptosis at high frequency in HeLa and other epithelial and fibroblast cell lines. This apoptotic phenotype in the infected cells was determined by several criteria including (i) visual changes in cell morphology, (ii) induction of chromatin condensation and nuclear marginalization, (iii) the presence of a high percentage of cells with subG1 DNA content, and (iv) activation of caspase-3 activity. Induction of the type III secretion machinery, but not invasion of P. aeruginosa is required for induction of apoptosis. The apoptosis phenotype is independent of the cytoskeletal rearrangements that occur in the host cell early after infection. Mutants in P. aeruginosa exoS fail to induce apoptosis and complementation with wild-type exoS restored the apoptosis-inducing capacity, demonstrating that ExoS is the effector molecule. Analysis of exoS activity mutants shows that the ADP-ribosylating capacity of ExoS is essential for inducing the apoptotic pathway.

Keywords: ExoS, apoptosis, type III secretion, ADP-ribosylation

Abbreviations: FCS, foetal calf serum; p.i., post infection; PI, propidium iodide; TNF{alpha}, tumour necrosis factor {alpha}




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
A. R. Hauser
Pseudomonas aeruginosa: An Uninvited Guest Refuses to Leave
Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 438 - 439.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. A. El Solh, M. E. Akinnusi, J. P. Wiener-Kronish, S. V. Lynch, L. A. Pineda, and K. Szarpa
Persistent Infection with Pseudomonas aeruginosa in Ventilator-associated Pneumonia
Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 513 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H. Yang, Z. Shan, J. Kim, W. Wu, W. Lian, L. Zeng, L. Xing, and S. Jin
Regulatory Role of PopN and Its Interacting Partners in Type III Secretion of Pseudomonas aeruginosa
J. Bacteriol., April 1, 2007; 189(7): 2599 - 2609.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Jia, Y. Wang, L. Zhou, and S. Jin
Expression of Pseudomonas aeruginosa Toxin ExoS Effectively Induces Apoptosis in Host Cells
Infect. Immun., December 1, 2006; 74(12): 6557 - 6570.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. P. O'Grady, H. Mulcahy, J. O'Callaghan, C. Adams, and F. O'Gara
Pseudomonas aeruginosa Infection of Airway Epithelial Cells Modulates Expression of Kruppel-Like Factors 2 and 6 via RsmA-Mediated Regulation of Type III Exoenzymes S and Y.
Infect. Immun., October 1, 2006; 74(10): 5893 - 5902.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. K. Shen, D. Filopon, L. Kuhn, B. Polack, and B. Toussaint
PsrA Is a Positive Transcriptional Regulator of the Type III Secretion System in Pseudomonas aeruginosa
Infect. Immun., February 1, 2006; 74(2): 1121 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
C. M. Shaver and A. R. Hauser
Interactions between effector proteins of the Pseudomonas aeruginosa type III secretion system do not significantly affect several measures of disease severity in mammals
Microbiology, January 1, 2006; 152(1): 143 - 152.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. Kim, K. Ahn, S. Min, J. Jia, U. Ha, D. Wu, and S. Jin
Factors triggering type III secretion in Pseudomonas aeruginosa
Microbiology, November 1, 2005; 151(11): 3575 - 3587.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W. Wu and S. Jin
PtrB of Pseudomonas aeruginosa Suppresses the Type III Secretion System under the Stress of DNA Damage
J. Bacteriol., September 1, 2005; 187(17): 6058 - 6068.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. Zhou, F. Lu, C. Latham, D. S. Zander, and G. A. Visner
Heme Oxygenase-1 Expression in Human Lungs with Cystic Fibrosis and Cytoprotective Effects against Pseudomonas Aeruginosa In Vitro
Am. J. Respir. Crit. Care Med., September 15, 2004; 170(6): 633 - 640.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
K.-S. Ahn, U. Ha, J. Jia, D. Wu, and S. Jin
The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes
Microbiology, March 1, 2004; 150(3): 539 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Tateda, Y. Ishii, M. Horikawa, T. Matsumoto, S. Miyairi, J. C. Pechere, T. J. Standiford, M. Ishiguro, and K. Yamaguchi
The Pseudomonas aeruginosa Autoinducer N-3-Oxododecanoyl Homoserine Lactone Accelerates Apoptosis in Macrophages and Neutrophils
Infect. Immun., October 1, 2003; 71(10): 5785 - 5793.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. J. Lee, B. A. Cowell, D. J. Evans, and S. M. J. Fleiszig
Contribution of ExsA-Regulated Factors to Corneal Infection by Cytotoxic and Invasive Pseudomonas aeruginosa in a Murine Scarification Model
Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 3892 - 3898.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Jia, M. Alaoui-El-Azher, M. Chow, T. C. Chambers, H. Baker, and S. Jin
c-Jun NH2-Terminal Kinase-Mediated Signaling Is Essential for Pseudomonas aeruginosa ExoS-Induced Apoptosis
Infect. Immun., June 1, 2003; 71(6): 3361 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Miyata, M. Casey, D. W. Frank, F. M. Ausubel, and E. Drenkard
Use of the Galleria mellonella Caterpillar as a Model Host To Study the Role of the Type III Secretion System in Pseudomonas aeruginosa Pathogenesis
Infect. Immun., May 1, 2003; 71(5): 2404 - 2413.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. Lory and J. K. Ichikawa
Pseudomonas-Epithelial Cell Interactions Dissected With DNA Microarrays
Chest, March 1, 2002; 121(2007): 36S - 40S.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
U. Ha and S. Jin
Growth Phase-Dependent Invasion of Pseudomonas aeruginosa and Its Survival within HeLa Cells
Infect. Immun., July 1, 2001; 69(7): 4398 - 4406.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 Society for General Microbiology.