| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Systematics and Evolution |
Departament de Microbiologia i Parasitologia Sanitàries, Divisió de Ciències de la Salut, Facultat de Farmàcia, Universitat de Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain1
Author for correspondence: J. G. Lorén. Tel: +34 93 402 44 97. Fax: +34 93 402 18 96. e-mail: loren{at}farmacia.far.ub.es
A total of 107 isolates of Vibrio cholerae, including 29 strains belonging to serogroup O139, were studied using multilocus enzyme electrophoresis (MLEE) to determine allelic variation in 15 housekeeping enzyme loci. All loci were polymorphic and 99 electrophoretic types (ETs) were identified from the total sample. No significant clustering of isolates was detected in the dendrogram generated from a matrix of coefficients of distances with respect to serogroup, biotype or country of isolation. The mean genetic diversity of this V. cholerae population (H=0·50) was higher than reported previously. Linkage disequilibrium analysis of the MLEE data showed a clonal structure for the entire population, but not in some of the population subgroups studied. This suggests an epidemic population structure. The results showed that the O139 strains were not clustered in a unique ET, in contrast to previous MLEE studies. This higher genetic variation of the O139 serogroup is concordant with ribotyping studies. The results also confirm that the O139 and O1 ElTor isolates are genetically more closely related to each other than to all the other subpopulations of V. cholerae studied.
Keywords: MLEE, linkage disequilibrium, cholera, population genetics, electrophoretic types
Abbreviations: AFLP, amplified fragment length polymorphism; ET, electrophoretic type; MLEE, multilocus enzyme electrophoresis; RAPD, random amplified polymorphic DNA
This article has been cited by other articles:
|
|
 |
|
  M. C. Miller, D. P. Keymer, A. Avelar, A. B. Boehm, and G. K. Schoolnik Detection and Transformation of Genome Segments That Differ within a Coastal Population of Vibrio cholerae Strains Appl. Envir. Microbiol., June 1, 2007; 73(11): 3695 - 3704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. A. O'Shea, F. J. Reen, A. M. Quirke, and E. F. Boyd Evolutionary Genetic Analysis of the Emergence of Epidemic Vibrio cholerae Isolates on the Basis of Comparative Nucleotide Sequence Analysis and Multilocus Virulence Gene Profiles J. Clin. Microbiol., October 1, 2004; 42(10): 4657 - 4671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L. Thompson, T. Iida, and J. Swings Biodiversity of Vibrios Microbiol. Mol. Biol. Rev., September 1, 2004; 68(3): 403 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Scortichini, E. Natalini, and L. Angelucci Clonal population structure of Pseudomonas avellanae strains of different origin based on multilocus enzyme electrophoresis Microbiology, October 1, 2003; 149(10): 2891 - 2900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Thompson, C. C. Thompson, A. C. P. Vicente, G. N. D. Theophilo, E. Hofer, and J. Swings Genomic Diversity of Clinical and Environmental Vibrio cholerae Strains Isolated in Brazil between 1991 and 2001 as Revealed by Fluorescent Amplified Fragment Length Polymorphism Analysis J. Clin. Microbiol., May 1, 2003; 41(5): 1946 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kotetishvili, O. C. Stine, Y. Chen, A. Kreger, A. Sulakvelidze, S. Sozhamannan, and J. G. Morris Jr. Multilocus Sequence Typing Has Better Discriminatory Ability for Typing Vibrio cholerae than Does Pulsed-Field Gel Electrophoresis and Provides a Measure of Phylogenetic Relatedness J. Clin. Microbiol., May 1, 2003; 41(5): 2191 - 2196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Faruque, D. A. Sack, R. B. Sack, R. R. Colwell, Y. Takeda, and G. B. Nair Inaugural Article: Emergence and evolution of Vibrio cholerae O139 PNAS, February 4, 2003; 100(3): 1304 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-G. Zo, I. N. G. Rivera, E. Russek-Cohen, M. S. Islam, A. K. Siddique, M. Yunus, R. B. Sack, A. Huq, and R. R. Colwell Genomic profiles of clinical and environmental isolates of Vibrio cholerae O1 in cholera-endemic areas of Bangladesh PNAS, September 17, 2002; 99(19): 12409 - 12414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Oberreuter, J. Charzinski, and S. Scherer Intraspecific diversity of Brevibacterium linens, Corynebacterium glutamicum and Rhodococcus erythropolis based on partial 16S rDNA sequence analysis and Fourier-transform infrared (FT-IR) spectroscopy Microbiology, May 1, 2002; 148(5): 1523 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Farfan, D. Minana-Galbis, M. C. Fuste, and J. G. Loren Allelic Diversity and Population Structure in Vibrio cholerae O139 Bengal Based on Nucleotide Sequence Analysis J. Bacteriol., March 1, 2002; 184(5): 1304 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rius, M. C. Fusté, C. Guasp, J. Lalucat, and J. G. Lorén Clonal Population Structure of Pseudomonas stutzeri, a Species with Exceptional Genetic Diversity J. Bacteriol., January 15, 2001; 183(2): 736 - 744. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
